Sonochemically prepared BSA microspheres containing Gemcitabine, and their potential application in renal cancer therapeutics

Olga Grinberg, Aharon Gedanken, Chitta Ranjan Patra, Sujata Patra, Priyabrata Mukherjee, Debabrata Mukhopadhyay

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

This report demonstrates the formation and characterization of sonochemically prepared bovine serum albumin (BSA)-Gemzar (Gemcitabine) microspheres and shows their increased anticancer activity compared to pristine Gemzar. The amount of loaded Gemzar was determined by light absorption measurements. The BSA-Gemzar composite was analyzed and characterized by optical microscopy and scanning electron microscopy. The release kinetics of Gemzar from the proteinaceous microspheres was tested. The BSA-Gemzar composite was examined for its anticancer activity (in vitro) in renal cancer cells (RCC, 786-O cells) using [3H]thymidine incorporation assays. It was found that the influence of the Gemzar-loaded microspheres on the cancer cells was significantly greater than that of an equimolar concentration of pristine Gemzar.

Original languageEnglish (US)
Pages (from-to)3031-3037
Number of pages7
JournalActa Biomaterialia
Volume5
Issue number8
DOIs
StatePublished - Oct 1 2009

Keywords

  • BSA microspheres
  • Drug delivery
  • Gemcitabine
  • Renal cancer 786-O cells
  • Sonochemistry

ASJC Scopus subject areas

  • Biotechnology
  • Biomaterials
  • Biochemistry
  • Biomedical Engineering
  • Molecular Biology

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