Somatostatin analogue octreotide modulates metabolism and effects of 5-fluorouracil and 5-fluorouridine in human colon cancer spheroids

Tong Bin Chen, Miljenko Huzak, Slobodan Macura, Stanimir Vuk-Pavlović

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

To examine if preferential retention of somatostatin analogues observed in some tumors might be used for modulation of effects of cancer drugs by co-treatment with long acting somatostatin analogues, the effects of somatostatin analogue octreotide on the kinetics of 5-fluorouracil (FUra) and 5-fluorouridine (FUrd) metabolism were studied by 19F NMR spectroscopy in multicell tumor spheroids comprised of human colon HT-29 adenocarcinoma cells. Octreotide stimulated the rat of formation of fluorouridinephosphates in FUra-treated cells, but inhibited this rate in FUrd-treated cells. Other elements of fluoropyrimidine metabolism were also altered by co-incubation with octreotide. A flow cytometric analysis indicated that FUra and FUrd arrested cells in the S phase, but co-treatment with octreotide almost eliminated the S-phase cells and induced the appearance of DNA fragments. These observations raise the possibility that somatostatin analogues can be used for specific modulation of fluoropyrimidine effects in tumors bearing somatostatin receptors.

Original languageEnglish (US)
Pages (from-to)41-51
Number of pages11
JournalCancer Letters
Volume86
Issue number1
DOIs
StatePublished - Oct 28 1994

Keywords

  • 5-Fluorouracil
  • 5-Fluorouridine
  • Colon adenocarcinoma HT-29 cells
  • Multicell spheroids
  • NMR spectroscopy
  • Octreotide

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Fingerprint Dive into the research topics of 'Somatostatin analogue octreotide modulates metabolism and effects of 5-fluorouracil and 5-fluorouridine in human colon cancer spheroids'. Together they form a unique fingerprint.

  • Cite this