Somatic cell mapping, polymorphism, and linkage analysis of bovine prolactin-related proteins and placental lactogen

Allan B. Dietz, Michel Georges, David W. Threadgill, James E. Womack, Linda A. Schuler

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

The bovine prolactin gene family includes novel members expressed in the fetal placenta that are distinct from placental lactogen. In this study, we investigated the genetic organization of four members of this gene family (PRP1, PRP3, PRP6, and PRP10) as well as placental lactogen (PL). Using a bovine-rodent hybrid somatic cell panel, all five genes were assigned to bovine chromosome 23, which contains prolactin and the major histocompatability group (BOLA). Restriction fragment length polymorphisms were detected by all probes in breeding populations with the restriction enzyme MspI, whereas no polymorphisms were detected with BamHI. EcoRI, HindIII, TaqI, and PstI produced polymorphic fragments with some but not all of the probes tested. A PRP10 polymorphism, which is apparently the result of a insertion/deletion event, detected polymorphism frequency differences between Bos indicus and Bos taurus. No recombinational events were observed with these probes and prolactin using linkage analysis involving 91 American Holsteins. The bovine prolactin gene family was incorporated into a linkage group containing CYP21. Our studies demonstrate that members of the bovine prolactin gene family have a close physical association with each other, and all members demonstrate genetic variability in the breeding population.

Original languageEnglish (US)
Pages (from-to)137-143
Number of pages7
JournalGenomics
Volume14
Issue number1
DOIs
StatePublished - Sep 1992

ASJC Scopus subject areas

  • Genetics

Fingerprint Dive into the research topics of 'Somatic cell mapping, polymorphism, and linkage analysis of bovine prolactin-related proteins and placental lactogen'. Together they form a unique fingerprint.

  • Cite this