Solvent-induced differentiation of protein backbone hydrogen bonds in calmodulin

Nenad Juranić, Elena Atanasova, John H. Streiff, Slobodan I Macura, Franklyn G. Prendergast

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

In apo and holoCaM, almost half of the hydrogen bonds (H-bonds) at the protein backbone expected from the corresponding NMR or X-ray structures were not detected by h3JNC′ couplings. The paucity of the h3JNC′ couplings was considered in terms of dynamic features of these structures. We examined a set of seven proteins and found that protein-backbone H-bonds form two groups according to the h3JNC′ couplings measured in solution. H-bonds that have h3JNC′ couplings above the threshold of 0.2 Hz show distance/angle correlation among the H-bond geometrical parameters, and appear to be supported by the backbone dynamics in solution. The other H-bonds have no such correlation; they populate the water-exposed and flexible regions of proteins, including many of the CaM helices. The observed differentiation in a dynamical behavior of backbone H-bonds in apo and holoCaM appears to be related to protein functions. Published by Cold Spring Harbor Laboratory Press.

Original languageEnglish (US)
Pages (from-to)1329-1337
Number of pages9
JournalProtein Science
Volume16
Issue number7
DOIs
StatePublished - Jul 2007

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Calmodulin
Hydrogen
Hydrogen bonds
Proteins
Ports and harbors
Nuclear magnetic resonance
X-Rays
X rays
Water

Keywords

  • J couplings
  • Calmodulin
  • Hydrogen bonding
  • Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Solvent-induced differentiation of protein backbone hydrogen bonds in calmodulin. / Juranić, Nenad; Atanasova, Elena; Streiff, John H.; Macura, Slobodan I; Prendergast, Franklyn G.

In: Protein Science, Vol. 16, No. 7, 07.2007, p. 1329-1337.

Research output: Contribution to journalArticle

Juranić, N, Atanasova, E, Streiff, JH, Macura, SI & Prendergast, FG 2007, 'Solvent-induced differentiation of protein backbone hydrogen bonds in calmodulin', Protein Science, vol. 16, no. 7, pp. 1329-1337. https://doi.org/10.1110/ps.062689807
Juranić, Nenad ; Atanasova, Elena ; Streiff, John H. ; Macura, Slobodan I ; Prendergast, Franklyn G. / Solvent-induced differentiation of protein backbone hydrogen bonds in calmodulin. In: Protein Science. 2007 ; Vol. 16, No. 7. pp. 1329-1337.
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