Solution measurement of DNA curvature in papillomavirus E2 binding sites

Jeff M. Zimmerman, L. James Maher

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

'Indirect readout' refers to the proposal that proteins can recognize the intrinsic three-dimensional shape or flexibility of a DNA binding sequence apart from direct protein contact with DNA base pairs. The differing affinities of human papillomavirus (HPV) E2 proteins for different E2 binding sites have been proposed to reflect indirect readout. DNA bending has been observed in X-ray structures of E2 protein-DNA complexes. X-ray structures of three different E2 DNA binding sites revealed differences in intrinsic curvature. DNA sites with intrinsic curvature in the direction of protein-induced bending were bound more tightly by E2 proteins, supporting the indirect readout model. We now report solution measurements of intrinsic DNA curvature for three E2 binding sites using a sensitive electrophoretic phasing assay. Measured E2 site curvature agrees well the predictions of a dinucleotide model and supports an indirect readout hypothesis for DNA recognition by HPV E2.

Original languageEnglish (US)
Pages (from-to)5134-5139
Number of pages6
JournalNucleic acids research
Volume31
Issue number17
DOIs
StatePublished - Sep 1 2003

ASJC Scopus subject areas

  • Genetics

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