Soluble Glycoprotein Is Not Required for Ebola Virus Virulence in Guinea Pigs

Thomas Hoenen; Andrea Marzi; Dana P. Scott; Friederike Feldmann; Julie Callison; David Safronetz; Hideki Ebihara; Heinz Feldmann

doi:10.1093/infdis/jiv111

Soluble Glycoprotein Is Not Required for Ebola Virus Virulence in Guinea Pigs

Thomas Hoenen, Andrea Marzi, Dana P. Scott, Friederike Feldmann, Julie Callison, David Safronetz, Hideki Ebihara, Heinz Feldmann

Molecular Medicine

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Ebola virus (EBOV) uses transcriptional editing to express several glycoproteins (GPs), including secreted soluble GP (sGP) and structural GP_1,2, from a single gene. Recombinant viruses predominantly expressing GP_1,2 are known to rapidly mutate and acquire an editing site predominantly expressing sGP in vivo, suggesting an important role of this protein during infection. Therefore, we generated a recombinant virus that is no longer able to express sGP and assessed its virulence in the EBOV Guinea pig model. Surprisingly, although this virus remained genetically stable, it did not show any significant attenuation in vivo, showing that sGP is not required for virulence in this model.

Original language	English (US)
Pages (from-to)	S242-S246
Journal	Journal of Infectious Diseases
Volume	212
DOIs	https://doi.org/10.1093/infdis/jiv111
State	Published - Oct 1 2015

Keywords

Guinea pigs
ebola virus
mRNA editing
recombinant virus
reverse genetics
sGP
soluble glycoprotein
virulence

ASJC Scopus subject areas

Immunology and Allergy
Infectious Diseases

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10.1093/infdis/jiv111

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title = "Soluble Glycoprotein Is Not Required for Ebola Virus Virulence in Guinea Pigs",

abstract = "Ebola virus (EBOV) uses transcriptional editing to express several glycoproteins (GPs), including secreted soluble GP (sGP) and structural GP1,2, from a single gene. Recombinant viruses predominantly expressing GP1,2 are known to rapidly mutate and acquire an editing site predominantly expressing sGP in vivo, suggesting an important role of this protein during infection. Therefore, we generated a recombinant virus that is no longer able to express sGP and assessed its virulence in the EBOV Guinea pig model. Surprisingly, although this virus remained genetically stable, it did not show any significant attenuation in vivo, showing that sGP is not required for virulence in this model.",

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AU - Feldmann, Friederike

AU - Callison, Julie

AU - Safronetz, David

AU - Ebihara, Hideki

AU - Feldmann, Heinz

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AB - Ebola virus (EBOV) uses transcriptional editing to express several glycoproteins (GPs), including secreted soluble GP (sGP) and structural GP1,2, from a single gene. Recombinant viruses predominantly expressing GP1,2 are known to rapidly mutate and acquire an editing site predominantly expressing sGP in vivo, suggesting an important role of this protein during infection. Therefore, we generated a recombinant virus that is no longer able to express sGP and assessed its virulence in the EBOV Guinea pig model. Surprisingly, although this virus remained genetically stable, it did not show any significant attenuation in vivo, showing that sGP is not required for virulence in this model.

KW - Guinea pigs

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KW - mRNA editing

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KW - reverse genetics

KW - sGP

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Soluble Glycoprotein Is Not Required for Ebola Virus Virulence in Guinea Pigs

Abstract

Keywords

ASJC Scopus subject areas

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