Soluble Glycoprotein Is Not Required for Ebola Virus Virulence in Guinea Pigs

Thomas Hoenen, Andrea Marzi, Dana P. Scott, Friederike Feldmann, Julie Callison, David Safronetz, Hideki Ebihara, Heinz Feldmann

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Ebola virus (EBOV) uses transcriptional editing to express several glycoproteins (GPs), including secreted soluble GP (sGP) and structural GP1,2, from a single gene. Recombinant viruses predominantly expressing GP1,2 are known to rapidly mutate and acquire an editing site predominantly expressing sGP in vivo, suggesting an important role of this protein during infection. Therefore, we generated a recombinant virus that is no longer able to express sGP and assessed its virulence in the EBOV Guinea pig model. Surprisingly, although this virus remained genetically stable, it did not show any significant attenuation in vivo, showing that sGP is not required for virulence in this model.

Original languageEnglish (US)
Pages (from-to)S242-S246
JournalJournal of Infectious Diseases
Volume212
DOIs
StatePublished - Jan 1 2015
Externally publishedYes

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Keywords

  • ebola virus
  • Guinea pigs
  • mRNA editing
  • recombinant virus
  • reverse genetics
  • sGP
  • soluble glycoprotein
  • virulence

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

Cite this

Hoenen, T., Marzi, A., Scott, D. P., Feldmann, F., Callison, J., Safronetz, D., Ebihara, H., & Feldmann, H. (2015). Soluble Glycoprotein Is Not Required for Ebola Virus Virulence in Guinea Pigs. Journal of Infectious Diseases, 212, S242-S246. https://doi.org/10.1093/infdis/jiv111