Abstract
Ebola virus (EBOV) uses transcriptional editing to express several glycoproteins (GPs), including secreted soluble GP (sGP) and structural GP1,2, from a single gene. Recombinant viruses predominantly expressing GP1,2 are known to rapidly mutate and acquire an editing site predominantly expressing sGP in vivo, suggesting an important role of this protein during infection. Therefore, we generated a recombinant virus that is no longer able to express sGP and assessed its virulence in the EBOV Guinea pig model. Surprisingly, although this virus remained genetically stable, it did not show any significant attenuation in vivo, showing that sGP is not required for virulence in this model.
| Original language | English (US) |
|---|---|
| Pages (from-to) | S242-S246 |
| Journal | Journal of Infectious Diseases |
| Volume | 212 |
| DOIs | |
| State | Published - Jan 1 2015 |
| Externally published | Yes |
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Keywords
- ebola virus
- Guinea pigs
- mRNA editing
- recombinant virus
- reverse genetics
- sGP
- soluble glycoprotein
- virulence
ASJC Scopus subject areas
- Immunology and Allergy
- Infectious Diseases
Cite this
Soluble Glycoprotein Is Not Required for Ebola Virus Virulence in Guinea Pigs. / Hoenen, Thomas; Marzi, Andrea; Scott, Dana P.; Feldmann, Friederike; Callison, Julie; Safronetz, David; Ebihara, Hideki; Feldmann, Heinz.
In: Journal of Infectious Diseases, Vol. 212, 01.01.2015, p. S242-S246.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Soluble Glycoprotein Is Not Required for Ebola Virus Virulence in Guinea Pigs
AU - Hoenen, Thomas
AU - Marzi, Andrea
AU - Scott, Dana P.
AU - Feldmann, Friederike
AU - Callison, Julie
AU - Safronetz, David
AU - Ebihara, Hideki
AU - Feldmann, Heinz
PY - 2015/1/1
Y1 - 2015/1/1
N2 - Ebola virus (EBOV) uses transcriptional editing to express several glycoproteins (GPs), including secreted soluble GP (sGP) and structural GP1,2, from a single gene. Recombinant viruses predominantly expressing GP1,2 are known to rapidly mutate and acquire an editing site predominantly expressing sGP in vivo, suggesting an important role of this protein during infection. Therefore, we generated a recombinant virus that is no longer able to express sGP and assessed its virulence in the EBOV Guinea pig model. Surprisingly, although this virus remained genetically stable, it did not show any significant attenuation in vivo, showing that sGP is not required for virulence in this model.
AB - Ebola virus (EBOV) uses transcriptional editing to express several glycoproteins (GPs), including secreted soluble GP (sGP) and structural GP1,2, from a single gene. Recombinant viruses predominantly expressing GP1,2 are known to rapidly mutate and acquire an editing site predominantly expressing sGP in vivo, suggesting an important role of this protein during infection. Therefore, we generated a recombinant virus that is no longer able to express sGP and assessed its virulence in the EBOV Guinea pig model. Surprisingly, although this virus remained genetically stable, it did not show any significant attenuation in vivo, showing that sGP is not required for virulence in this model.
KW - ebola virus
KW - Guinea pigs
KW - mRNA editing
KW - recombinant virus
KW - reverse genetics
KW - sGP
KW - soluble glycoprotein
KW - virulence
UR - http://www.scopus.com/inward/record.url?scp=84942862617&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84942862617&partnerID=8YFLogxK
U2 - 10.1093/infdis/jiv111
DO - 10.1093/infdis/jiv111
M3 - Article
C2 - 25957965
AN - SCOPUS:84942862617
VL - 212
SP - S242-S246
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
SN - 0022-1899
ER -