Soluble Glycoprotein Is Not Required for Ebola Virus Virulence in Guinea Pigs

Thomas Hoenen; Andrea Marzi; Dana P. Scott; Friederike Feldmann; Julie Callison; David Safronetz; Hideki Ebihara; Heinz Feldmann

doi:10.1093/infdis/jiv111

Soluble Glycoprotein Is Not Required for Ebola Virus Virulence in Guinea Pigs

Thomas Hoenen, Andrea Marzi, Dana P. Scott, Friederike Feldmann, Julie Callison, David Safronetz, Hideki Ebihara, Heinz Feldmann

Research output: Contribution to journal › Article

10 Scopus citations

Abstract

Ebola virus (EBOV) uses transcriptional editing to express several glycoproteins (GPs), including secreted soluble GP (sGP) and structural GP_1,2, from a single gene. Recombinant viruses predominantly expressing GP_1,2 are known to rapidly mutate and acquire an editing site predominantly expressing sGP in vivo, suggesting an important role of this protein during infection. Therefore, we generated a recombinant virus that is no longer able to express sGP and assessed its virulence in the EBOV Guinea pig model. Surprisingly, although this virus remained genetically stable, it did not show any significant attenuation in vivo, showing that sGP is not required for virulence in this model.

Original language	English (US)
Pages (from-to)	S242-S246
Journal	Journal of Infectious Diseases
Volume	212
DOIs	https://doi.org/10.1093/infdis/jiv111
State	Published - Jan 1 2015
Externally published	Yes

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Keywords

ebola virus
Guinea pigs
mRNA editing
recombinant virus
reverse genetics
sGP
soluble glycoprotein
virulence

ASJC Scopus subject areas

Immunology and Allergy
Infectious Diseases

Cite this

Hoenen, T., Marzi, A., Scott, D. P., Feldmann, F., Callison, J., Safronetz, D., Ebihara, H., & Feldmann, H. (2015). Soluble Glycoprotein Is Not Required for Ebola Virus Virulence in Guinea Pigs. Journal of Infectious Diseases, 212, S242-S246. https://doi.org/10.1093/infdis/jiv111

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Access to Document

10.1093/infdis/jiv111