Soluble derivatives of the β amyloid protein precursor of Alzheimer's disease are labeled by antisera to the β amyloid protein

Mark R. Palmert, Sandra L. Siedlak, Marcia Berman Podlisny, Barry Greenberg, Earl R. Shelton, Hardy W. Chan, Marianne Usiak, Dennis J. Selkbe, George Perry, Steven G Younkin

Research output: Contribution to journalArticle

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Abstract

The amyloid deposited in Alzheimer's disease (AD) is composed primarily of a 39-42 residue polypeptide (βAP) that is derived from a larger β amyloid protein precursor (βAPP). In previous studies, we and others identified full-length, membrane-associated forms of the βAPP and showed that these forms are processed into soluble derivatives that lack the carboxyl-terminus of the full-length forms. In this report, we demonstrate that the soluble ∼125 and ∼105 kDa forms of the βAPP found in human cerebrospinal fluid are specifically labeled by several different antisera to the βAP. This finding indicates that both soluble derivatives contain all or part of the βAP sequence, and it suggests that one or both of these forms may be the immediate precursor of the amyloid deposited in AD.

Original languageEnglish (US)
Pages (from-to)182-188
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume165
Issue number1
DOIs
StatePublished - Nov 30 1989
Externally publishedYes

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Amyloidogenic Proteins
Amyloid beta-Protein Precursor
Immune Sera
Alzheimer Disease
Derivatives
Amyloid
Cerebrospinal fluid
Cerebrospinal Fluid
Membranes
Peptides

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Soluble derivatives of the β amyloid protein precursor of Alzheimer's disease are labeled by antisera to the β amyloid protein. / Palmert, Mark R.; Siedlak, Sandra L.; Podlisny, Marcia Berman; Greenberg, Barry; Shelton, Earl R.; Chan, Hardy W.; Usiak, Marianne; Selkbe, Dennis J.; Perry, George; Younkin, Steven G.

In: Biochemical and Biophysical Research Communications, Vol. 165, No. 1, 30.11.1989, p. 182-188.

Research output: Contribution to journalArticle

Palmert, Mark R. ; Siedlak, Sandra L. ; Podlisny, Marcia Berman ; Greenberg, Barry ; Shelton, Earl R. ; Chan, Hardy W. ; Usiak, Marianne ; Selkbe, Dennis J. ; Perry, George ; Younkin, Steven G. / Soluble derivatives of the β amyloid protein precursor of Alzheimer's disease are labeled by antisera to the β amyloid protein. In: Biochemical and Biophysical Research Communications. 1989 ; Vol. 165, No. 1. pp. 182-188.
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AU - Shelton, Earl R.

AU - Chan, Hardy W.

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AU - Selkbe, Dennis J.

AU - Perry, George

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