SNIP1 Is a Candidate Modifier of the Transcriptional Activity of c-Myc on E Box-Dependent Target Genes

Makiko Fujii, Lyudmila A. Lyakh, Cameron P Bracken, Junya Fukuoka, Morisada Hayakawa, Tadasuke Tsukiyama, Steven J Soll, Melissa Harris, Sonia Rocha, Kevin C Roche, Shin ichi Tominaga, Jin Jen, Neil D Perkins, Robert J. Lechleider, Anita B Roberts

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Using a yeast two-hybrid screen, we found that SNIP1 (Smad nuclear-interacting protein 1) associates with c-Myc, a key regulator of cell proliferation and transformation. We demonstrate that SNIP1 functions as an important regulator of c-Myc activity, binding the N terminus of c-Myc through its own C terminus, and that SNIP1 enhances the transcriptional activity of c-Myc both by stabilizing it against proteosomal degradation and by bridging the c-Myc/p300 complex. These effects of SNIP1 on c-Myc likely contribute to synergistic effects of SNIP1, c-Myc, and H-Ras in inducing formation of foci in an in vitro transformation assay and also in supporting anchorage-independent growth. The significant association of SNIP1 and c-Myc staining in a non-small cell lung cancer tissue array is further evidence that their activities might be linked and suggests that SNIP1 might be an important modulator of c-Myc activity in carcinogenesis.

Original languageEnglish (US)
Pages (from-to)771-783
Number of pages13
JournalMolecular Cell
Volume24
Issue number5
DOIs
StatePublished - Dec 8 2006
Externally publishedYes

Fingerprint

Smad Proteins
Nuclear Proteins
Genes
Non-Small Cell Lung Carcinoma
Carcinogenesis
Yeasts
Cell Proliferation
Staining and Labeling
Growth

Keywords

  • CELLCYCLE
  • DNA

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Fujii, M., Lyakh, L. A., Bracken, CP., Fukuoka, J., Hayakawa, M., Tsukiyama, T., ... Roberts, AB. (2006). SNIP1 Is a Candidate Modifier of the Transcriptional Activity of c-Myc on E Box-Dependent Target Genes. Molecular Cell, 24(5), 771-783. https://doi.org/10.1016/j.molcel.2006.11.006

SNIP1 Is a Candidate Modifier of the Transcriptional Activity of c-Myc on E Box-Dependent Target Genes. / Fujii, Makiko; Lyakh, Lyudmila A.; Bracken, Cameron P; Fukuoka, Junya; Hayakawa, Morisada; Tsukiyama, Tadasuke; Soll, Steven J; Harris, Melissa; Rocha, Sonia; Roche, Kevin C; Tominaga, Shin ichi; Jen, Jin; Perkins, Neil D; Lechleider, Robert J.; Roberts, Anita B.

In: Molecular Cell, Vol. 24, No. 5, 08.12.2006, p. 771-783.

Research output: Contribution to journalArticle

Fujii, M, Lyakh, LA, Bracken, CP, Fukuoka, J, Hayakawa, M, Tsukiyama, T, Soll, SJ, Harris, M, Rocha, S, Roche, KC, Tominaga, SI, Jen, J, Perkins, ND, Lechleider, RJ & Roberts, AB 2006, 'SNIP1 Is a Candidate Modifier of the Transcriptional Activity of c-Myc on E Box-Dependent Target Genes', Molecular Cell, vol. 24, no. 5, pp. 771-783. https://doi.org/10.1016/j.molcel.2006.11.006
Fujii, Makiko ; Lyakh, Lyudmila A. ; Bracken, Cameron P ; Fukuoka, Junya ; Hayakawa, Morisada ; Tsukiyama, Tadasuke ; Soll, Steven J ; Harris, Melissa ; Rocha, Sonia ; Roche, Kevin C ; Tominaga, Shin ichi ; Jen, Jin ; Perkins, Neil D ; Lechleider, Robert J. ; Roberts, Anita B. / SNIP1 Is a Candidate Modifier of the Transcriptional Activity of c-Myc on E Box-Dependent Target Genes. In: Molecular Cell. 2006 ; Vol. 24, No. 5. pp. 771-783.
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