Abstract
Background: Ispinesib is a small molecule inhibitor of Eg5 and induces neck linker docking. Results: Ispinesib binding to Eg5 induces subsequent movement of switch II. Conclusion: Conformational changes in L5 can alter the orientation of the neck linker regardless of the state of the catalytic site. Significance: L5 is a key regulator of both the catalytic site and switch II.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 18588-18598 |
| Number of pages | 11 |
| Journal | Journal of Biological Chemistry |
| Volume | 288 |
| Issue number | 25 |
| DOIs | |
| State | Published - Jun 21 2013 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Cell Biology
Cite this
Snapshots of ispinesib-induced conformational changes in the mitotic kinesin eg5. / Kaan, Hung Yi Kristal; Major, Jennifer; Tkocz, Katarzyna; Kozielski, Frank; Rosenfeld, Steven.
In: Journal of Biological Chemistry, Vol. 288, No. 25, 21.06.2013, p. 18588-18598.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Snapshots of ispinesib-induced conformational changes in the mitotic kinesin eg5
AU - Kaan, Hung Yi Kristal
AU - Major, Jennifer
AU - Tkocz, Katarzyna
AU - Kozielski, Frank
AU - Rosenfeld, Steven
PY - 2013/6/21
Y1 - 2013/6/21
N2 - Background: Ispinesib is a small molecule inhibitor of Eg5 and induces neck linker docking. Results: Ispinesib binding to Eg5 induces subsequent movement of switch II. Conclusion: Conformational changes in L5 can alter the orientation of the neck linker regardless of the state of the catalytic site. Significance: L5 is a key regulator of both the catalytic site and switch II.
AB - Background: Ispinesib is a small molecule inhibitor of Eg5 and induces neck linker docking. Results: Ispinesib binding to Eg5 induces subsequent movement of switch II. Conclusion: Conformational changes in L5 can alter the orientation of the neck linker regardless of the state of the catalytic site. Significance: L5 is a key regulator of both the catalytic site and switch II.
UR - http://www.scopus.com/inward/record.url?scp=84880052495&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84880052495&partnerID=8YFLogxK
U2 - 10.1074/jbc.M113.462648
DO - 10.1074/jbc.M113.462648
M3 - Article
C2 - 23658017
AN - SCOPUS:84880052495
VL - 288
SP - 18588
EP - 18598
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 25
ER -