Snapshots of ispinesib-induced conformational changes in the mitotic kinesin eg5

Hung Yi Kristal Kaan; Jennifer Major; Katarzyna Tkocz; Frank Kozielski; Steven S. Rosenfeld

doi:10.1074/jbc.M113.462648

Snapshots of ispinesib-induced conformational changes in the mitotic kinesin eg5

Hung Yi Kristal Kaan, Jennifer Major, Katarzyna Tkocz, Frank Kozielski, Steven S. Rosenfeld

Hematology / Oncology / Cancer Center / Breast Clinic

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Background: Ispinesib is a small molecule inhibitor of Eg5 and induces neck linker docking. Results: Ispinesib binding to Eg5 induces subsequent movement of switch II. Conclusion: Conformational changes in L5 can alter the orientation of the neck linker regardless of the state of the catalytic site. Significance: L5 is a key regulator of both the catalytic site and switch II.

Original language	English (US)
Pages (from-to)	18588-18598
Number of pages	11
Journal	Journal of Biological Chemistry
Volume	288
Issue number	25
DOIs	https://doi.org/10.1074/jbc.M113.462648
State	Published - Jun 21 2013

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Cell Biology

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abstract = "Background: Ispinesib is a small molecule inhibitor of Eg5 and induces neck linker docking. Results: Ispinesib binding to Eg5 induces subsequent movement of switch II. Conclusion: Conformational changes in L5 can alter the orientation of the neck linker regardless of the state of the catalytic site. Significance: L5 is a key regulator of both the catalytic site and switch II.",

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AU - Rosenfeld, Steven S.

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