Small heat shock proteins inhibit in vitro Aβ1-42 amyloidogenesis

Yogish C Kudva, Henry J. Hiddinga, Peter C. Butler, Cheryl S. Mueske, Norman L. Eberhardt

Research output: Contribution to journalArticle

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Abstract

We demonstrate that small heat shock proteins (sHsp) inhibit in vitro amyloid formation by the Alzheimer's Aβ1-42 polypeptide as detected by a thioflavine T fluorescence assay and electron microscopy. Human sHsp27 (0.50-3.0 μM) inhibited amyloid formation from 20 μM Aβ1-42 by 23-75% in 24 h. In contrast, treatment of pre-formed amyloid with 0.5-3.0 μM sHsp27 only reduced the fluorescence signal by 6-36%. The data suggest that ordered fibril formation may represent a form of off-pathway aggregation that can he prevented by chaperone action. The data raise the possibility that age-related changes in chaperone function could contribute toward the pathogenesis of Alzheimer's and other amyloid-associated diseases.

Original languageEnglish (US)
Pages (from-to)117-121
Number of pages5
JournalFEBS Letters
Volume416
Issue number1
DOIs
StatePublished - Oct 13 1997

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Small Heat-Shock Proteins
Amyloid
Fluorescence
Fluorescence Microscopy
Electron microscopy
Assays
Electron Microscopy
Agglomeration
Peptides
In Vitro Techniques

Keywords

  • Alzheimer's disease
  • Amyloidogenesis
  • Chaperone
  • Heat shock protein

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Kudva, Y. C., Hiddinga, H. J., Butler, P. C., Mueske, C. S., & Eberhardt, N. L. (1997). Small heat shock proteins inhibit in vitro Aβ1-42 amyloidogenesis. FEBS Letters, 416(1), 117-121. https://doi.org/10.1016/S0014-5793(97)01180-0

Small heat shock proteins inhibit in vitro Aβ1-42 amyloidogenesis. / Kudva, Yogish C; Hiddinga, Henry J.; Butler, Peter C.; Mueske, Cheryl S.; Eberhardt, Norman L.

In: FEBS Letters, Vol. 416, No. 1, 13.10.1997, p. 117-121.

Research output: Contribution to journalArticle

Kudva, YC, Hiddinga, HJ, Butler, PC, Mueske, CS & Eberhardt, NL 1997, 'Small heat shock proteins inhibit in vitro Aβ1-42 amyloidogenesis', FEBS Letters, vol. 416, no. 1, pp. 117-121. https://doi.org/10.1016/S0014-5793(97)01180-0
Kudva, Yogish C ; Hiddinga, Henry J. ; Butler, Peter C. ; Mueske, Cheryl S. ; Eberhardt, Norman L. / Small heat shock proteins inhibit in vitro Aβ1-42 amyloidogenesis. In: FEBS Letters. 1997 ; Vol. 416, No. 1. pp. 117-121.
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