Skin regeneration with all accessory organs following ablation with irreversible electroporation

Alexander Golberg, Martin Villiger, G. Felix Broelsch, Kyle P. Quinn, Hassan Albadawi, Saiqa Khan, Michael T. Watkins, Irene Georgakoudi, William G. Austen, Marianna Bei, Brett E. Bouma, Martin C. Mihm, Martin L. Yarmush

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Skin scar formation is a complex process that results in the formation of dense extracellular matrix (ECM) without normal skin appendages such as hair and glands. The absence of a scarless healing model in adult mammals prevents the development of successful therapies. We show that irreversible electroporation of skin drives its regeneration with all accessory organs in normal adult rats. Pulsed electric fields at 500 V, with 70 μs pulse duration and 1000 pulses delivered at 3 Hz, applied through two electrodes separated by 2 mm lead to massive cell death. However, the ECM architecture of the skin was preserved. Six months after the ablation, the epidermis, sebaceous glands, panniculus carnosus, hair follicles, microvasculature and arrector pili muscle were altogether re-formed in the entire ablated area. These results suggest a key role of the ECM architecture in the differentiation, migration and signalling of cells during scarless wound healing.

Original languageEnglish (US)
Pages (from-to)98-113
Number of pages16
JournalJournal of Tissue Engineering and Regenerative Medicine
Volume12
Issue number1
DOIs
StatePublished - Jan 1 2018

Fingerprint

Electroporation
Accessories
Ablation
Regeneration
Skin
Extracellular Matrix
Sebaceous Glands
Mammals
Hair Follicle
Cell death
Microvessels
Epidermis
Wound Healing
Hair
Cell Movement
Cicatrix
Muscle
Rats
Electrodes
Cell Death

Keywords

  • electroporation
  • extracellular matrix
  • pulsed electric field
  • regeneration
  • scar
  • scarless
  • skin

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering

Cite this

Skin regeneration with all accessory organs following ablation with irreversible electroporation. / Golberg, Alexander; Villiger, Martin; Felix Broelsch, G.; Quinn, Kyle P.; Albadawi, Hassan; Khan, Saiqa; Watkins, Michael T.; Georgakoudi, Irene; Austen, William G.; Bei, Marianna; Bouma, Brett E.; Mihm, Martin C.; Yarmush, Martin L.

In: Journal of Tissue Engineering and Regenerative Medicine, Vol. 12, No. 1, 01.01.2018, p. 98-113.

Research output: Contribution to journalArticle

Golberg, A, Villiger, M, Felix Broelsch, G, Quinn, KP, Albadawi, H, Khan, S, Watkins, MT, Georgakoudi, I, Austen, WG, Bei, M, Bouma, BE, Mihm, MC & Yarmush, ML 2018, 'Skin regeneration with all accessory organs following ablation with irreversible electroporation', Journal of Tissue Engineering and Regenerative Medicine, vol. 12, no. 1, pp. 98-113. https://doi.org/10.1002/term.2374
Golberg, Alexander ; Villiger, Martin ; Felix Broelsch, G. ; Quinn, Kyle P. ; Albadawi, Hassan ; Khan, Saiqa ; Watkins, Michael T. ; Georgakoudi, Irene ; Austen, William G. ; Bei, Marianna ; Bouma, Brett E. ; Mihm, Martin C. ; Yarmush, Martin L. / Skin regeneration with all accessory organs following ablation with irreversible electroporation. In: Journal of Tissue Engineering and Regenerative Medicine. 2018 ; Vol. 12, No. 1. pp. 98-113.
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