Abstract
Skin scar formation is a complex process that results in the formation of dense extracellular matrix (ECM) without normal skin appendages such as hair and glands. The absence of a scarless healing model in adult mammals prevents the development of successful therapies. We show that irreversible electroporation of skin drives its regeneration with all accessory organs in normal adult rats. Pulsed electric fields at 500 V, with 70 μs pulse duration and 1000 pulses delivered at 3 Hz, applied through two electrodes separated by 2 mm lead to massive cell death. However, the ECM architecture of the skin was preserved. Six months after the ablation, the epidermis, sebaceous glands, panniculus carnosus, hair follicles, microvasculature and arrector pili muscle were altogether re-formed in the entire ablated area. These results suggest a key role of the ECM architecture in the differentiation, migration and signalling of cells during scarless wound healing.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 98-113 |
| Number of pages | 16 |
| Journal | Journal of Tissue Engineering and Regenerative Medicine |
| Volume | 12 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1 2018 |
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Keywords
- electroporation
- extracellular matrix
- pulsed electric field
- regeneration
- scar
- scarless
- skin
ASJC Scopus subject areas
- Medicine (miscellaneous)
- Biomaterials
- Biomedical Engineering
Cite this
Skin regeneration with all accessory organs following ablation with irreversible electroporation. / Golberg, Alexander; Villiger, Martin; Felix Broelsch, G.; Quinn, Kyle P.; Albadawi, Hassan; Khan, Saiqa; Watkins, Michael T.; Georgakoudi, Irene; Austen, William G.; Bei, Marianna; Bouma, Brett E.; Mihm, Martin C.; Yarmush, Martin L.
In: Journal of Tissue Engineering and Regenerative Medicine, Vol. 12, No. 1, 01.01.2018, p. 98-113.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Skin regeneration with all accessory organs following ablation with irreversible electroporation
AU - Golberg, Alexander
AU - Villiger, Martin
AU - Felix Broelsch, G.
AU - Quinn, Kyle P.
AU - Albadawi, Hassan
AU - Khan, Saiqa
AU - Watkins, Michael T.
AU - Georgakoudi, Irene
AU - Austen, William G.
AU - Bei, Marianna
AU - Bouma, Brett E.
AU - Mihm, Martin C.
AU - Yarmush, Martin L.
PY - 2018/1/1
Y1 - 2018/1/1
N2 - Skin scar formation is a complex process that results in the formation of dense extracellular matrix (ECM) without normal skin appendages such as hair and glands. The absence of a scarless healing model in adult mammals prevents the development of successful therapies. We show that irreversible electroporation of skin drives its regeneration with all accessory organs in normal adult rats. Pulsed electric fields at 500 V, with 70 μs pulse duration and 1000 pulses delivered at 3 Hz, applied through two electrodes separated by 2 mm lead to massive cell death. However, the ECM architecture of the skin was preserved. Six months after the ablation, the epidermis, sebaceous glands, panniculus carnosus, hair follicles, microvasculature and arrector pili muscle were altogether re-formed in the entire ablated area. These results suggest a key role of the ECM architecture in the differentiation, migration and signalling of cells during scarless wound healing.
AB - Skin scar formation is a complex process that results in the formation of dense extracellular matrix (ECM) without normal skin appendages such as hair and glands. The absence of a scarless healing model in adult mammals prevents the development of successful therapies. We show that irreversible electroporation of skin drives its regeneration with all accessory organs in normal adult rats. Pulsed electric fields at 500 V, with 70 μs pulse duration and 1000 pulses delivered at 3 Hz, applied through two electrodes separated by 2 mm lead to massive cell death. However, the ECM architecture of the skin was preserved. Six months after the ablation, the epidermis, sebaceous glands, panniculus carnosus, hair follicles, microvasculature and arrector pili muscle were altogether re-formed in the entire ablated area. These results suggest a key role of the ECM architecture in the differentiation, migration and signalling of cells during scarless wound healing.
KW - electroporation
KW - extracellular matrix
KW - pulsed electric field
KW - regeneration
KW - scar
KW - scarless
KW - skin
UR - http://www.scopus.com/inward/record.url?scp=85019592572&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85019592572&partnerID=8YFLogxK
U2 - 10.1002/term.2374
DO - 10.1002/term.2374
M3 - Article
C2 - 27976527
AN - SCOPUS:85019592572
VL - 12
SP - 98
EP - 113
JO - Journal of Tissue Engineering and Regenerative Medicine
JF - Journal of Tissue Engineering and Regenerative Medicine
SN - 1932-6254
IS - 1
ER -