@article{4e73731734e048a880b9084e762b1160,
title = "Skin regeneration with all accessory organs following ablation with irreversible electroporation",
abstract = "Skin scar formation is a complex process that results in the formation of dense extracellular matrix (ECM) without normal skin appendages such as hair and glands. The absence of a scarless healing model in adult mammals prevents the development of successful therapies. We show that irreversible electroporation of skin drives its regeneration with all accessory organs in normal adult rats. Pulsed electric fields at 500 V, with 70 μs pulse duration and 1000 pulses delivered at 3 Hz, applied through two electrodes separated by 2 mm lead to massive cell death. However, the ECM architecture of the skin was preserved. Six months after the ablation, the epidermis, sebaceous glands, panniculus carnosus, hair follicles, microvasculature and arrector pili muscle were altogether re-formed in the entire ablated area. These results suggest a key role of the ECM architecture in the differentiation, migration and signalling of cells during scarless wound healing.",
keywords = "electroporation, extracellular matrix, pulsed electric field, regeneration, scar, scarless, skin",
author = "Alexander Golberg and Martin Villiger and {Felix Broelsch}, G. and Quinn, {Kyle P.} and Hassan Albadawi and Saiqa Khan and Watkins, {Michael T.} and Irene Georgakoudi and Austen, {William G.} and Marianna Bei and Bouma, {Brett E.} and Mihm, {Martin C.} and Yarmush, {Martin L.}",
note = "Funding Information: The authors acknowledge Shriners Grant #85120-BOS and Bionational USA-Israel Science Foundation (BSF) for the support of this study BSF Grant # 2015286. Research in this publication was also supported in part by the Department of Defense, Air Force Office of Scientific Research under agreement number FA9550-13-1-0068, and the National Institute of Biomedical Imaging and Bioengineering of the National Institutes of Health, award P41 EB015903. Electron microscopy was performed in the Microscopy Core of the Center for Systems Biology/Program in Membrane Biology, which is partially supported by an Inflammatory Bowel Disease Grant DK43351 and a Boston Area Diabetes and Endocrinology Research Center (BADERC) Award DK57521. The authors thank Dana-Farber/Harvard Cancer Center in Boston, MA, for the use of the Rodent Histopathology Core, which provided histopathology service. Dana-Farber/Harvard Cancer Center is supported in part by NCI Cancer Center Support Grant # NIH 5 P30 CA06516.",
year = "2018",
month = jan,
doi = "10.1002/term.2374",
language = "English (US)",
volume = "12",
pages = "98--113",
journal = "Journal of Tissue Engineering and Regenerative Medicine",
issn = "1932-6254",
publisher = "John Wiley and Sons Ltd",
number = "1",
}