@article{0e0522aa17f14f3f9a886b6f6a2f75af,
title = "Skeletal muscles of mice deficient in muscle creatine kinase lack burst activity",
abstract = "To understand the physiological role of the creatine kinase-phosphocreatine (CK-PCr) system in muscle bioenergetics, a null mutation of the muscle CK (M-CK) gene was introduced into the germline of mice. Mutant mice show no alterations in absolute muscle force, but lack the ability to perform burst activity. Their fast-twitch fibers have an increased intermyofibrillar mitochondrial volume and an increased glycogenolytic/glycolytic potential. PCr and ATP levels are normal in resting M-CK-deficient muscles, but rates of high energy phosphate exchange between PCr and ATP are at least 20-fold reduced. Strikingly, PCr levels decline normally during muscle exercise, suggesting that M-CK-mediated conversion is not the only route for PCr utilization in active muscle.",
author = "{van Deursen}, Jan and Arend Heerschap and Frank Oerlemans and Wim Rultenbeek and Paul Jap and {ter Laak}, Henk and B{\'e} Wieringa",
note = "Funding Information: The authors wish to thank Wilma Peters, Jan Schepens, Mietske Wijers-Rouw, Eric van de Boogert, Theo van Lith, Antoon Janssen, and Henny Kuppen for their excellent technical assistance. We are grateful to Hans Degens and Rob Binkhorst of the Department of Physiology for help and useful discussions in measurements of muscle force. We also thank Janine van Ree, David Iles, Edwin Mariman, and Wiljan Hendriks for critically reading the manuscript; Robin Lovell-Badge for advice on ES cell microinjection; Allan Bradley for supplying the AB-1 ES cells; and our colleagues of the Central Animal Facility for help and advice. This work was supported by a program grant from the Dutch Organization for Scientific Research (Medical Sciences) and carried out at the NMR facilities of the Department of Biophysical Chemistry, University of Nijmegen, with support of the Dutch Organization for Scientific Research (Chemical Sciences).",
year = "1993",
month = aug,
day = "27",
doi = "10.1016/0092-8674(93)90510-W",
language = "English (US)",
volume = "74",
pages = "621--631",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "4",
}