Skeletal muscles of mice deficient in muscle creatine kinase lack burst activity

Jan Van Deursen, Arend Heerschap, Frank Oerlemans, Wim Rultenbeek, Paul Jap, Henk ter Laak, Bé Wieringa

Research output: Contribution to journalArticle

258 Citations (Scopus)

Abstract

To understand the physiological role of the creatine kinase-phosphocreatine (CK-PCr) system in muscle bioenergetics, a null mutation of the muscle CK (M-CK) gene was introduced into the germline of mice. Mutant mice show no alterations in absolute muscle force, but lack the ability to perform burst activity. Their fast-twitch fibers have an increased intermyofibrillar mitochondrial volume and an increased glycogenolytic/glycolytic potential. PCr and ATP levels are normal in resting M-CK-deficient muscles, but rates of high energy phosphate exchange between PCr and ATP are at least 20-fold reduced. Strikingly, PCr levels decline normally during muscle exercise, suggesting that M-CK-mediated conversion is not the only route for PCr utilization in active muscle.

Original languageEnglish (US)
Pages (from-to)621-631
Number of pages11
JournalCell
Volume74
Issue number4
DOIs
StatePublished - Aug 27 1993
Externally publishedYes

Fingerprint

MM Form Creatine Kinase
Muscle
Skeletal Muscle
Muscles
Adenosine Triphosphate
Mitochondrial Size
Phosphocreatine
Creatine Kinase
Energy Metabolism
Genes
Phosphates
Mutation
Fibers

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Van Deursen, J., Heerschap, A., Oerlemans, F., Rultenbeek, W., Jap, P., ter Laak, H., & Wieringa, B. (1993). Skeletal muscles of mice deficient in muscle creatine kinase lack burst activity. Cell, 74(4), 621-631. https://doi.org/10.1016/0092-8674(93)90510-W

Skeletal muscles of mice deficient in muscle creatine kinase lack burst activity. / Van Deursen, Jan; Heerschap, Arend; Oerlemans, Frank; Rultenbeek, Wim; Jap, Paul; ter Laak, Henk; Wieringa, Bé.

In: Cell, Vol. 74, No. 4, 27.08.1993, p. 621-631.

Research output: Contribution to journalArticle

Van Deursen, J, Heerschap, A, Oerlemans, F, Rultenbeek, W, Jap, P, ter Laak, H & Wieringa, B 1993, 'Skeletal muscles of mice deficient in muscle creatine kinase lack burst activity', Cell, vol. 74, no. 4, pp. 621-631. https://doi.org/10.1016/0092-8674(93)90510-W
Van Deursen J, Heerschap A, Oerlemans F, Rultenbeek W, Jap P, ter Laak H et al. Skeletal muscles of mice deficient in muscle creatine kinase lack burst activity. Cell. 1993 Aug 27;74(4):621-631. https://doi.org/10.1016/0092-8674(93)90510-W
Van Deursen, Jan ; Heerschap, Arend ; Oerlemans, Frank ; Rultenbeek, Wim ; Jap, Paul ; ter Laak, Henk ; Wieringa, Bé. / Skeletal muscles of mice deficient in muscle creatine kinase lack burst activity. In: Cell. 1993 ; Vol. 74, No. 4. pp. 621-631.
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