Single-strand conformation polymorphisms (SSCPs) detected in five bovine genes.

H. L. Neibergs, Allan B Dietz, J. E. Womack

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Polymerase chain reaction (PCR) primers designed to amplify bovine specific sequences of the arginine-vasopressin (ARVP), glycoprotein hormone alpha (CGA), cytochrome oxidase c subunit IV pseudogene (COXP), prochymosin (CYM), coagulation factor X (F10), inhibin beta A (INHBA), low density lipoprotein receptor (LDLR) and oxytocin (OXT) genes in hybrid cells were used in a search for single strand conformation polymorphisms. DNA from 75 animals comprising crossbred and 7 purebred breeds were analysed. ARVP, COXP, CYM, LDLR and OXT were found to be polymorphic while CGA, F10 and INHBA were not. Polymorphic regions were identified within 206 bp of exon 1 of ARVP, 582 bp of the pseudogene COXP, 253 bp of exon 9 of CYM, 519 bp of LDLR cDNA and 160 bp of the upstream regulatory region of OXT. This is the first report of bovine polymorphisms for these genes and an important step in our goal to incorporate type I comparative anchor loci into the bovine linkage map. Polymorphic loci were subsequently analysed in pedigreed full-sib families and shown to be inherited in a Mendelian fashion.

Original languageEnglish (US)
Pages (from-to)81-84
Number of pages4
JournalAnimal Genetics
Volume24
Issue number2
StatePublished - Apr 1993
Externally publishedYes

Fingerprint

arginine vasopressin
single-stranded conformational polymorphism
Arginine Vasopressin
LDL Receptors
oxytocin
Oxytocin
low density lipoprotein
inhibin
Pseudogenes
pseudogenes
receptors
exons
Exons
cattle
Genes
Factor X
loci
genes
Hybrid Cells
Nucleic Acid Regulatory Sequences

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Genetics
  • Genetics(clinical)

Cite this

Single-strand conformation polymorphisms (SSCPs) detected in five bovine genes. / Neibergs, H. L.; Dietz, Allan B; Womack, J. E.

In: Animal Genetics, Vol. 24, No. 2, 04.1993, p. 81-84.

Research output: Contribution to journalArticle

Neibergs, H. L. ; Dietz, Allan B ; Womack, J. E. / Single-strand conformation polymorphisms (SSCPs) detected in five bovine genes. In: Animal Genetics. 1993 ; Vol. 24, No. 2. pp. 81-84.
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