Single-strand conformation polymorphism and heteroduplex analysis for gel-based mutation detection

Arun J. Nataraj, Isabelle Olivos-Glander, Noriko Kusukawa, W. Edward Highsmith

Research output: Contribution to journalArticlepeer-review

206 Scopus citations

Abstract

Single-strand conformation polymorphism (SSCP) and heteroduplex analysis (HA) are popular electrophoretic methods for the identification of sequences. The principle reasons for the popularity of these two methods are their technical simplicity and their relatively high sensitivity for the detection of mutations. Here we review the theory and practice of SSCP and HA, including the factors contributing to the sensitivity of mutation detection. For SSCP analysis, these factors include: choice of gel matrix, electrophoretic conditions, presence of neutral additives, fragment size, and G+C content. For HA, the principle factors influencing sensitivity are the gel matrix and the identity of the base mismatch.

Original languageEnglish (US)
Pages (from-to)1177-1185
Number of pages9
JournalELECTROPHORESIS
Volume20
Issue number6
DOIs
StatePublished - Jun 16 1999

Keywords

  • Heteroduplex analysis
  • Mutation detection
  • Review
  • Single-strand conformation polymorphism

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

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