Single molecule mechanical properties of type II collagen and hyaluronan measured by optical tweezers

Zong Ping Luo, Yu Long Sun, Tadashi Fujii, Kai Nan An

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Type II collagen and hyaluronan are the two major components of extracellular molecules in cartilage and play an important role in mechanical functions of extracellular matrix. Currently, their mechanical properties have been investigated only at the gross-level. In this study, the mechanical properties of single type II collagen and hyaluronan molecules were directly measured using optical tweezers technique. The persistence length was found to be 11.2±8.4 nm in type II collagen and 4.5 ± 1.2 nm in hyaluronan. This result suggested that type II collagen is suffer than hyaluronan at the individual molecule level, which supports the general concept that collagen is responsible for resisting tensile force. The experimental system developed here also provides a powerful tool for quantifying mechanical properties of extracellular matrix at the single molecule level.

Original languageEnglish (US)
Pages (from-to)247-254
Number of pages8
JournalBiorheology
Volume41
Issue number3-4
StatePublished - 2004

Fingerprint

Optical Tweezers
Collagen Type II
Hyaluronic Acid
Extracellular Matrix
Cartilage
Collagen

Keywords

  • Hyaluronan
  • Mechanical properties
  • Optical tweezers
  • Persistence length
  • Type II collagen

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Luo, Z. P., Sun, Y. L., Fujii, T., & An, K. N. (2004). Single molecule mechanical properties of type II collagen and hyaluronan measured by optical tweezers. Biorheology, 41(3-4), 247-254.

Single molecule mechanical properties of type II collagen and hyaluronan measured by optical tweezers. / Luo, Zong Ping; Sun, Yu Long; Fujii, Tadashi; An, Kai Nan.

In: Biorheology, Vol. 41, No. 3-4, 2004, p. 247-254.

Research output: Contribution to journalArticle

Luo, ZP, Sun, YL, Fujii, T & An, KN 2004, 'Single molecule mechanical properties of type II collagen and hyaluronan measured by optical tweezers', Biorheology, vol. 41, no. 3-4, pp. 247-254.
Luo, Zong Ping ; Sun, Yu Long ; Fujii, Tadashi ; An, Kai Nan. / Single molecule mechanical properties of type II collagen and hyaluronan measured by optical tweezers. In: Biorheology. 2004 ; Vol. 41, No. 3-4. pp. 247-254.
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