Single-molecule detection of H2O2 mediating angiogenic redox signaling on fluorescent single-walled carbon nanotube array

Jong Ho Kim, Chitta Ranjan Patra, Jyoti R. Arkalgud, Ardemis A. Boghossian, Jingqing Zhang, Jae Hee Han, Nigel F. Reuel, Jin Ho Ahn, Debabrata Mukhopadhyay, Michael S. Strano

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Reactive oxygen species, specifically hydrogen peroxide (H 2O2), activate signal transduction pathways during angiogenesis and therefore play an important role in physiological development as well as various pathophysiologies. Herein, we utilize a near-infrared fluorescent single-walled carbon nanotube (SWNT) sensor array to measure the single-molecule efflux of H2O2 from human umbilical vein endothelial cells (HUVEC) in response to angiogenic stimulation. Two angiogenic agents were investigated: the pro-angiogenic cytokine, vascular endothelial growth factor A (VEGF-A) and the recently identified inorganic pro-angiogenic factor, europium(III) hydroxide in nanorod form. The nanosensor array consists ofa SWNT embedded within a collagen matrix that exhibits high selectivity and sensitivity to single molecules of H2O2. A calibration from 12.5 to 400 nM quantifies the production of H2O2 at nanomolar concentration in HUVEC with 1 s temporal and 300 nm spatial resolutions. We find that the production of H2O2 following VEGF stimulation is elevated outside of HUVEC, but not for stimulation via nanorods, while increased generation is observed in the cytoplasm for both cases, suggesting two distinct signaling pathways.

Original languageEnglish (US)
Pages (from-to)7848-7857
Number of pages10
JournalACS Nano
Volume5
Issue number10
DOIs
StatePublished - Oct 25 2011

Fingerprint

Endothelial cells
Single-walled carbon nanotubes (SWCN)
stimulation
veins
carbon nanotubes
Nanorods
Vascular Endothelial Growth Factor A
nanorods
Molecules
Europium
Nanosensors
efflux
molecules
angiogenesis
Signal transduction
cytoplasm
Angiogenesis Inducing Agents
Sensor arrays
collagens
europium

Keywords

  • angiogenesis
  • europium(III) hydroxide nanorods
  • hydrogen peroxide
  • near-infrared fluorescence
  • redox signaling
  • single-molecule detection
  • single-walled carbon nanotube

ASJC Scopus subject areas

  • Engineering(all)
  • Materials Science(all)
  • Physics and Astronomy(all)

Cite this

Kim, J. H., Patra, C. R., Arkalgud, J. R., Boghossian, A. A., Zhang, J., Han, J. H., ... Strano, M. S. (2011). Single-molecule detection of H2O2 mediating angiogenic redox signaling on fluorescent single-walled carbon nanotube array. ACS Nano, 5(10), 7848-7857. https://doi.org/10.1021/nn201904t

Single-molecule detection of H2O2 mediating angiogenic redox signaling on fluorescent single-walled carbon nanotube array. / Kim, Jong Ho; Patra, Chitta Ranjan; Arkalgud, Jyoti R.; Boghossian, Ardemis A.; Zhang, Jingqing; Han, Jae Hee; Reuel, Nigel F.; Ahn, Jin Ho; Mukhopadhyay, Debabrata; Strano, Michael S.

In: ACS Nano, Vol. 5, No. 10, 25.10.2011, p. 7848-7857.

Research output: Contribution to journalArticle

Kim, JH, Patra, CR, Arkalgud, JR, Boghossian, AA, Zhang, J, Han, JH, Reuel, NF, Ahn, JH, Mukhopadhyay, D & Strano, MS 2011, 'Single-molecule detection of H2O2 mediating angiogenic redox signaling on fluorescent single-walled carbon nanotube array', ACS Nano, vol. 5, no. 10, pp. 7848-7857. https://doi.org/10.1021/nn201904t
Kim, Jong Ho ; Patra, Chitta Ranjan ; Arkalgud, Jyoti R. ; Boghossian, Ardemis A. ; Zhang, Jingqing ; Han, Jae Hee ; Reuel, Nigel F. ; Ahn, Jin Ho ; Mukhopadhyay, Debabrata ; Strano, Michael S. / Single-molecule detection of H2O2 mediating angiogenic redox signaling on fluorescent single-walled carbon nanotube array. In: ACS Nano. 2011 ; Vol. 5, No. 10. pp. 7848-7857.
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