Single-fraction irradiation has no effect on uptake of radiolabeled pegylated liposomes in a tumor xenograft model

K. J. Harrington, G. Rowlinson-Busza, P. S. Uster, Richard Geoffrey Vile, A. M. Peters, J. S W Stewart

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Purpose: These studies were performed with the intention of examining the effect of single-fraction doses of radiotherapy (RT) on the tumor deposition of radiolabeled pegylated liposomes in an animal xenograft tumor model. Methods and Materials: Human KB head-and-neck xenograft tumors were established in female nude mice. The effect of single fraction tumor RT doses (5, 10, 15, and 20 Gy) on the tumor uptake of intravenously administered 111In-DTPA-labeled pegylated liposomes (IDLPL) was examined using two protocols: (1) to test the effect of RT delivered 30 min before liposome injection on the time course of tumor uptake over a 96-h period; (2) to test the effect of RT at times ranging from 72-h to 1-h before liposome injection on the levels of liposome uptake at 24 h. Tumor and normal tissue/organ (blood, liver, spleen, lung, and kidney) liposome uptake was determined by dissection and quantitation in a gamma counter. Results: There was no demonstrable effect of RT on tumor uptake of IDLPL (p > 0.1 for all comparisons). Reassuringly, neither was there an effect of RT on the pharmacokinetics and biodistribution of radiolabeled liposomes to normal tissues. Conclusions: Single fraction doses of RT appear to have no effect on tumor or normal tissue biodistribution and pharmacokinetics of radiolabeled pegylated liposomes in this animal model.

Original languageEnglish (US)
Pages (from-to)1141-1148
Number of pages8
JournalInternational Journal of Radiation Oncology Biology Physics
Volume49
Issue number4
DOIs
StatePublished - Mar 15 2001

Fingerprint

Heterografts
Liposomes
tumors
radiation therapy
Radiotherapy
irradiation
Neoplasms
dosage
Pharmacokinetics
injection
dissection
Pentetic Acid
Injections
animal models
spleen
kidneys
liver
Nude Mice
organs
lungs

Keywords

  • Biodistribution
  • Chemoradiotherapy
  • Pegylated liposome
  • Xenograft

ASJC Scopus subject areas

  • Oncology
  • Radiology Nuclear Medicine and imaging
  • Radiation

Cite this

Single-fraction irradiation has no effect on uptake of radiolabeled pegylated liposomes in a tumor xenograft model. / Harrington, K. J.; Rowlinson-Busza, G.; Uster, P. S.; Vile, Richard Geoffrey; Peters, A. M.; Stewart, J. S W.

In: International Journal of Radiation Oncology Biology Physics, Vol. 49, No. 4, 15.03.2001, p. 1141-1148.

Research output: Contribution to journalArticle

Harrington, K. J. ; Rowlinson-Busza, G. ; Uster, P. S. ; Vile, Richard Geoffrey ; Peters, A. M. ; Stewart, J. S W. / Single-fraction irradiation has no effect on uptake of radiolabeled pegylated liposomes in a tumor xenograft model. In: International Journal of Radiation Oncology Biology Physics. 2001 ; Vol. 49, No. 4. pp. 1141-1148.
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abstract = "Purpose: These studies were performed with the intention of examining the effect of single-fraction doses of radiotherapy (RT) on the tumor deposition of radiolabeled pegylated liposomes in an animal xenograft tumor model. Methods and Materials: Human KB head-and-neck xenograft tumors were established in female nude mice. The effect of single fraction tumor RT doses (5, 10, 15, and 20 Gy) on the tumor uptake of intravenously administered 111In-DTPA-labeled pegylated liposomes (IDLPL) was examined using two protocols: (1) to test the effect of RT delivered 30 min before liposome injection on the time course of tumor uptake over a 96-h period; (2) to test the effect of RT at times ranging from 72-h to 1-h before liposome injection on the levels of liposome uptake at 24 h. Tumor and normal tissue/organ (blood, liver, spleen, lung, and kidney) liposome uptake was determined by dissection and quantitation in a gamma counter. Results: There was no demonstrable effect of RT on tumor uptake of IDLPL (p > 0.1 for all comparisons). Reassuringly, neither was there an effect of RT on the pharmacokinetics and biodistribution of radiolabeled liposomes to normal tissues. Conclusions: Single fraction doses of RT appear to have no effect on tumor or normal tissue biodistribution and pharmacokinetics of radiolabeled pegylated liposomes in this animal model.",
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