Single chamber DNA extraction and amplification for prosthetic joint infection detection

Lindsay A. Legendre, Weidong Cao, Kerryl Piper, Jerome P. Ferrance, Robin Patel, James P. Landers

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

This work reports on the application of a microchip system integrating DNA extraction with infrared-mediated (IR) PCR amplification within a single chamber for the detection of of bacterial infections within prosthetic joints. Results show not only the successful integration of DNA extraction and amplification in a single chamber, but an improvement over the PCR detection limit for bacterial infection when compared to conventional molecular biological methods.

Original languageEnglish (US)
Title of host publicationMicro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherJapan Academic Association Inc
Pages840-842
Number of pages3
StatePublished - 2006
Event10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan
Duration: Nov 5 2006Nov 9 2006

Other

Other10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
CountryJapan
CityTokyo
Period11/5/0611/9/06

Fingerprint

Prosthetics
Amplification
DNA
Infrared radiation

Keywords

  • Bacterial infection detection
  • DNA extraction
  • Microchip integration
  • PCR

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Legendre, L. A., Cao, W., Piper, K., Ferrance, J. P., Patel, R., & Landers, J. P. (2006). Single chamber DNA extraction and amplification for prosthetic joint infection detection. In Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 840-842). Japan Academic Association Inc.

Single chamber DNA extraction and amplification for prosthetic joint infection detection. / Legendre, Lindsay A.; Cao, Weidong; Piper, Kerryl; Ferrance, Jerome P.; Patel, Robin; Landers, James P.

Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Japan Academic Association Inc, 2006. p. 840-842.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Legendre, LA, Cao, W, Piper, K, Ferrance, JP, Patel, R & Landers, JP 2006, Single chamber DNA extraction and amplification for prosthetic joint infection detection. in Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Japan Academic Association Inc, pp. 840-842, 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006, Tokyo, Japan, 11/5/06.
Legendre LA, Cao W, Piper K, Ferrance JP, Patel R, Landers JP. Single chamber DNA extraction and amplification for prosthetic joint infection detection. In Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Japan Academic Association Inc. 2006. p. 840-842
Legendre, Lindsay A. ; Cao, Weidong ; Piper, Kerryl ; Ferrance, Jerome P. ; Patel, Robin ; Landers, James P. / Single chamber DNA extraction and amplification for prosthetic joint infection detection. Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Japan Academic Association Inc, 2006. pp. 840-842
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