Single chamber DNA extraction and amplification for prosthetic joint infection detection

Lindsay A. Legendre, Weidong Cao, Kerryl Piper, Jerome P. Ferrance, Robin Patel, James P. Landers

Research output: Contribution to conferencePaperpeer-review

2 Scopus citations

Abstract

This work reports on the application of a microchip system integrating DNA extraction with infrared-mediated (IR) PCR amplification within a single chamber for the detection of of bacterial infections within prosthetic joints. Results show not only the successful integration of DNA extraction and amplification in a single chamber, but an improvement over the PCR detection limit for bacterial infection when compared to conventional molecular biological methods.

Original languageEnglish (US)
Pages840-842
Number of pages3
StatePublished - Jan 1 2006
Event10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan
Duration: Nov 5 2006Nov 9 2006

Other

Other10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
CountryJapan
CityTokyo
Period11/5/0611/9/06

Keywords

  • Bacterial infection detection
  • DNA extraction
  • Microchip integration
  • PCR

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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