Single chamber DNA extraction and amplification for prosthetic joint infection detection

Lindsay A. Legendre; Weidong Cao; Kerryl Piper; Jerome P. Ferrance; Robin Patel; James P. Landers

Single chamber DNA extraction and amplification for prosthetic joint infection detection

Lindsay A. Legendre, Weidong Cao, Kerryl Piper, Jerome P. Ferrance, Robin Patel, James P. Landers

Laboratory Medicine and Pathology

Research output: Contribution to conference › Paper › peer-review

2 Scopus citations

Abstract

This work reports on the application of a microchip system integrating DNA extraction with infrared-mediated (IR) PCR amplification within a single chamber for the detection of of bacterial infections within prosthetic joints. Results show not only the successful integration of DNA extraction and amplification in a single chamber, but an improvement over the PCR detection limit for bacterial infection when compared to conventional molecular biological methods.

Original language	English (US)
Pages	840-842
Number of pages	3
State	Published - Jan 1 2006
Event	10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan Duration: Nov 5 2006 → Nov 9 2006

Other

Other	10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
Country/Territory	Japan
City	Tokyo
Period	11/5/06 → 11/9/06

Keywords

Bacterial infection detection
DNA extraction
Microchip integration
PCR

ASJC Scopus subject areas

Chemical Engineering (miscellaneous)
Bioengineering

Cite this

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title = "Single chamber DNA extraction and amplification for prosthetic joint infection detection",

abstract = "This work reports on the application of a microchip system integrating DNA extraction with infrared-mediated (IR) PCR amplification within a single chamber for the detection of of bacterial infections within prosthetic joints. Results show not only the successful integration of DNA extraction and amplification in a single chamber, but an improvement over the PCR detection limit for bacterial infection when compared to conventional molecular biological methods.",

keywords = "Bacterial infection detection, DNA extraction, Microchip integration, PCR",

author = "Legendre, {Lindsay A.} and Weidong Cao and Kerryl Piper and Ferrance, {Jerome P.} and Robin Patel and Landers, {James P.}",

year = "2006",

month = jan,

day = "1",

language = "English (US)",

pages = "840--842",

note = "10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 ; Conference date: 05-11-2006 Through 09-11-2006",

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TY - CONF

T1 - Single chamber DNA extraction and amplification for prosthetic joint infection detection

AU - Legendre, Lindsay A.

AU - Cao, Weidong

AU - Piper, Kerryl

AU - Ferrance, Jerome P.

AU - Patel, Robin

AU - Landers, James P.

PY - 2006/1/1

Y1 - 2006/1/1

N2 - This work reports on the application of a microchip system integrating DNA extraction with infrared-mediated (IR) PCR amplification within a single chamber for the detection of of bacterial infections within prosthetic joints. Results show not only the successful integration of DNA extraction and amplification in a single chamber, but an improvement over the PCR detection limit for bacterial infection when compared to conventional molecular biological methods.

AB - This work reports on the application of a microchip system integrating DNA extraction with infrared-mediated (IR) PCR amplification within a single chamber for the detection of of bacterial infections within prosthetic joints. Results show not only the successful integration of DNA extraction and amplification in a single chamber, but an improvement over the PCR detection limit for bacterial infection when compared to conventional molecular biological methods.

KW - Bacterial infection detection

KW - DNA extraction

KW - Microchip integration

KW - PCR

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UR - http://www.scopus.com/inward/citedby.url?scp=84902457216&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:84902457216

SP - 840

EP - 842

T2 - 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006

Y2 - 5 November 2006 through 9 November 2006

ER -

Single chamber DNA extraction and amplification for prosthetic joint infection detection

Abstract

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Keywords

ASJC Scopus subject areas

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