Single-cell nonphotochemical hole burning of ovarian surface epithelial carcinoma and normal cells

R. J. Walsh, S. Matsuzaki, T. Reinot, J. M. Hayes, K. R. Kalli, L. C. Hartmann, G. J. Small

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Persistent spectral nonphotochemical hole-burning (NPHB) spectroscopy has recently been applied to dye molecules in cells. The sensitivity of NPHB to the nanoenvironment of the probe is well established. It has been shown that NPHB applied to bulk suspensions of cultured human cells can distinguish between normal and cancer cells. Thus, NPHB has potential as a diagnostic cancer tool. For this reason, the methodology is referred to as hole-burning imaging, by analogy with MRI. The optical dephasing time (T2) of the dye in hole-burning image replaces the proton T1 relaxation time in MRI. In addition to the T2 mode of operation, there are four other modes including measurement of the spectral hole growth kinetics (HGK). Reported here is that the selectivity and sensitivity of NPHB operating in the HGK mode allow for distinction between normal and carcinoma cells at the single-cell level. The ovarian cell lines are ovarian surface epithelial cells with temperature-sensitive large T antigens (analogously normal) and ovarian surface epithelial carcinoma (OV167) cells. The mitochondrial specific dye used was rhodamine 800 (Molecular Probes). This carbocationic dye is highly specific for the outer and inner membranes of mitochondria. In line with the results for bulk suspensions of the two cell lines, the hole-burning efficiency for OV167 cells was found to be significantly higher than that for normal cells. Theoretical analysis of the HGK data leads to the conclusion that the degree of structural heterogeneity for the probe-host configurations in OV167 cells is lower than in the normal cells. Possible reasons for this are given.

Original languageEnglish (US)
Pages (from-to)1685-1689
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume100
Issue number4
DOIs
StatePublished - Feb 18 2003

Fingerprint

Carcinoma
Coloring Agents
Suspensions
Growth
Molecular Probes
Cell Line
Viral Tumor Antigens
Protons
Cultured Cells
Neoplasms
Spectrum Analysis
Mitochondria
Epithelial Cells
Temperature
Membranes

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Single-cell nonphotochemical hole burning of ovarian surface epithelial carcinoma and normal cells. / Walsh, R. J.; Matsuzaki, S.; Reinot, T.; Hayes, J. M.; Kalli, K. R.; Hartmann, L. C.; Small, G. J.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, No. 4, 18.02.2003, p. 1685-1689.

Research output: Contribution to journalArticle

Walsh, R. J. ; Matsuzaki, S. ; Reinot, T. ; Hayes, J. M. ; Kalli, K. R. ; Hartmann, L. C. ; Small, G. J. / Single-cell nonphotochemical hole burning of ovarian surface epithelial carcinoma and normal cells. In: Proceedings of the National Academy of Sciences of the United States of America. 2003 ; Vol. 100, No. 4. pp. 1685-1689.
@article{cb9e09a28caf4c38ac3609f9b1189cde,
title = "Single-cell nonphotochemical hole burning of ovarian surface epithelial carcinoma and normal cells",
abstract = "Persistent spectral nonphotochemical hole-burning (NPHB) spectroscopy has recently been applied to dye molecules in cells. The sensitivity of NPHB to the nanoenvironment of the probe is well established. It has been shown that NPHB applied to bulk suspensions of cultured human cells can distinguish between normal and cancer cells. Thus, NPHB has potential as a diagnostic cancer tool. For this reason, the methodology is referred to as hole-burning imaging, by analogy with MRI. The optical dephasing time (T2) of the dye in hole-burning image replaces the proton T1 relaxation time in MRI. In addition to the T2 mode of operation, there are four other modes including measurement of the spectral hole growth kinetics (HGK). Reported here is that the selectivity and sensitivity of NPHB operating in the HGK mode allow for distinction between normal and carcinoma cells at the single-cell level. The ovarian cell lines are ovarian surface epithelial cells with temperature-sensitive large T antigens (analogously normal) and ovarian surface epithelial carcinoma (OV167) cells. The mitochondrial specific dye used was rhodamine 800 (Molecular Probes). This carbocationic dye is highly specific for the outer and inner membranes of mitochondria. In line with the results for bulk suspensions of the two cell lines, the hole-burning efficiency for OV167 cells was found to be significantly higher than that for normal cells. Theoretical analysis of the HGK data leads to the conclusion that the degree of structural heterogeneity for the probe-host configurations in OV167 cells is lower than in the normal cells. Possible reasons for this are given.",
author = "Walsh, {R. J.} and S. Matsuzaki and T. Reinot and Hayes, {J. M.} and Kalli, {K. R.} and Hartmann, {L. C.} and Small, {G. J.}",
year = "2003",
month = "2",
day = "18",
doi = "10.1073/pnas.0437668100",
language = "English (US)",
volume = "100",
pages = "1685--1689",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "4",

}

TY - JOUR

T1 - Single-cell nonphotochemical hole burning of ovarian surface epithelial carcinoma and normal cells

AU - Walsh, R. J.

AU - Matsuzaki, S.

AU - Reinot, T.

AU - Hayes, J. M.

AU - Kalli, K. R.

AU - Hartmann, L. C.

AU - Small, G. J.

PY - 2003/2/18

Y1 - 2003/2/18

N2 - Persistent spectral nonphotochemical hole-burning (NPHB) spectroscopy has recently been applied to dye molecules in cells. The sensitivity of NPHB to the nanoenvironment of the probe is well established. It has been shown that NPHB applied to bulk suspensions of cultured human cells can distinguish between normal and cancer cells. Thus, NPHB has potential as a diagnostic cancer tool. For this reason, the methodology is referred to as hole-burning imaging, by analogy with MRI. The optical dephasing time (T2) of the dye in hole-burning image replaces the proton T1 relaxation time in MRI. In addition to the T2 mode of operation, there are four other modes including measurement of the spectral hole growth kinetics (HGK). Reported here is that the selectivity and sensitivity of NPHB operating in the HGK mode allow for distinction between normal and carcinoma cells at the single-cell level. The ovarian cell lines are ovarian surface epithelial cells with temperature-sensitive large T antigens (analogously normal) and ovarian surface epithelial carcinoma (OV167) cells. The mitochondrial specific dye used was rhodamine 800 (Molecular Probes). This carbocationic dye is highly specific for the outer and inner membranes of mitochondria. In line with the results for bulk suspensions of the two cell lines, the hole-burning efficiency for OV167 cells was found to be significantly higher than that for normal cells. Theoretical analysis of the HGK data leads to the conclusion that the degree of structural heterogeneity for the probe-host configurations in OV167 cells is lower than in the normal cells. Possible reasons for this are given.

AB - Persistent spectral nonphotochemical hole-burning (NPHB) spectroscopy has recently been applied to dye molecules in cells. The sensitivity of NPHB to the nanoenvironment of the probe is well established. It has been shown that NPHB applied to bulk suspensions of cultured human cells can distinguish between normal and cancer cells. Thus, NPHB has potential as a diagnostic cancer tool. For this reason, the methodology is referred to as hole-burning imaging, by analogy with MRI. The optical dephasing time (T2) of the dye in hole-burning image replaces the proton T1 relaxation time in MRI. In addition to the T2 mode of operation, there are four other modes including measurement of the spectral hole growth kinetics (HGK). Reported here is that the selectivity and sensitivity of NPHB operating in the HGK mode allow for distinction between normal and carcinoma cells at the single-cell level. The ovarian cell lines are ovarian surface epithelial cells with temperature-sensitive large T antigens (analogously normal) and ovarian surface epithelial carcinoma (OV167) cells. The mitochondrial specific dye used was rhodamine 800 (Molecular Probes). This carbocationic dye is highly specific for the outer and inner membranes of mitochondria. In line with the results for bulk suspensions of the two cell lines, the hole-burning efficiency for OV167 cells was found to be significantly higher than that for normal cells. Theoretical analysis of the HGK data leads to the conclusion that the degree of structural heterogeneity for the probe-host configurations in OV167 cells is lower than in the normal cells. Possible reasons for this are given.

UR - http://www.scopus.com/inward/record.url?scp=0037452609&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037452609&partnerID=8YFLogxK

U2 - 10.1073/pnas.0437668100

DO - 10.1073/pnas.0437668100

M3 - Article

VL - 100

SP - 1685

EP - 1689

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 4

ER -