Single-Cell and Population-Level Analyses Using Real-Time Kinetic Labeling Couples Proliferation and Cell Death Mechanisms

Jesse D. Gelles; Jarvier N. Mohammed; Luis C. Santos; Diana Legarda; Adrian T. Ting; Jerry E. Chipuk

doi:10.1016/j.devcel.2019.08.016

Single-Cell and Population-Level Analyses Using Real-Time Kinetic Labeling Couples Proliferation and Cell Death Mechanisms

Jesse D. Gelles, Jarvier N. Mohammed, Luis C. Santos, Diana Legarda, Adrian T. Ting, Jerry E. Chipuk

Immunology

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

To quantify cell death in high-throughput studies, Gelles et al. develop a robust method for single-cell and population-level analyses using real-time kinetic labeling (SPARKL). Example protocols and mathematical analyses detail the characterization of cell death kinetics and mechanisms, with coupled changes to proliferation, for use within high-volume comparative approaches.

Original language	English (US)
Pages (from-to)	277-291.e4
Journal	Developmental Cell
Volume	51
Issue number	2
DOIs	https://doi.org/10.1016/j.devcel.2019.08.016
State	Published - Oct 21 2019

Keywords

apoptosis
automated image analysis
cell death
ferroptosis
high-throughput
live-cell imaging
necroptosis
real-time assay

ASJC Scopus subject areas

Molecular Biology
General Biochemistry, Genetics and Molecular Biology
Developmental Biology
Cell Biology

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10.1016/j.devcel.2019.08.016

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title = "Single-Cell and Population-Level Analyses Using Real-Time Kinetic Labeling Couples Proliferation and Cell Death Mechanisms",

abstract = "To quantify cell death in high-throughput studies, Gelles et al. develop a robust method for single-cell and population-level analyses using real-time kinetic labeling (SPARKL). Example protocols and mathematical analyses detail the characterization of cell death kinetics and mechanisms, with coupled changes to proliferation, for use within high-volume comparative approaches.",

keywords = "apoptosis, automated image analysis, cell death, ferroptosis, high-throughput, live-cell imaging, necroptosis, real-time assay",

author = "Gelles, {Jesse D.} and Mohammed, {Jarvier N.} and Santos, {Luis C.} and Diana Legarda and Ting, {Adrian T.} and Chipuk, {Jerry E.}",

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AU - Gelles, Jesse D.

AU - Mohammed, Jarvier N.

AU - Santos, Luis C.

AU - Legarda, Diana

AU - Ting, Adrian T.

AU - Chipuk, Jerry E.

PY - 2019/10/21

Y1 - 2019/10/21

N2 - To quantify cell death in high-throughput studies, Gelles et al. develop a robust method for single-cell and population-level analyses using real-time kinetic labeling (SPARKL). Example protocols and mathematical analyses detail the characterization of cell death kinetics and mechanisms, with coupled changes to proliferation, for use within high-volume comparative approaches.

AB - To quantify cell death in high-throughput studies, Gelles et al. develop a robust method for single-cell and population-level analyses using real-time kinetic labeling (SPARKL). Example protocols and mathematical analyses detail the characterization of cell death kinetics and mechanisms, with coupled changes to proliferation, for use within high-volume comparative approaches.

KW - apoptosis

KW - automated image analysis

KW - cell death

KW - ferroptosis

KW - high-throughput

KW - live-cell imaging

KW - necroptosis

KW - real-time assay

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JF - Developmental Cell

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ER -

Single-Cell and Population-Level Analyses Using Real-Time Kinetic Labeling Couples Proliferation and Cell Death Mechanisms

Abstract

Keywords

ASJC Scopus subject areas

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