Single-Cell and Population-Level Analyses Using Real-Time Kinetic Labeling Couples Proliferation and Cell Death Mechanisms

Jesse D. Gelles, Jarvier N. Mohammed, Luis C. Santos, Diana Legarda, Adrian T. Ting, Jerry E. Chipuk

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

To quantify cell death in high-throughput studies, Gelles et al. develop a robust method for single-cell and population-level analyses using real-time kinetic labeling (SPARKL). Example protocols and mathematical analyses detail the characterization of cell death kinetics and mechanisms, with coupled changes to proliferation, for use within high-volume comparative approaches.

Original languageEnglish (US)
Pages (from-to)277-291.e4
JournalDevelopmental Cell
Volume51
Issue number2
DOIs
StatePublished - Oct 21 2019

Keywords

  • apoptosis
  • automated image analysis
  • cell death
  • ferroptosis
  • high-throughput
  • live-cell imaging
  • necroptosis
  • real-time assay

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Developmental Biology
  • Cell Biology

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