Single and combinatorial chromatin coupling events underlies the function of transcript factor krüppel-like factor 11 in the regulation of gene networks

Ezequiel Calvo, Adrienne Grzenda, Gwen Lomberk, Angela Mathison, Juan Iovanna, Raul Urrutia

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Background: Krüppel-like factors (KLFs) are a group of master regulators of gene expression conserved from flies to human. However, scant information is available on either the mechanisms or functional impact of the coupling of KLF proteins to chromatin remodeling machines, a deterministic step in transcriptional regulation.Results and discussion: In the current study, we use genome-wide analyses of chromatin immunoprecipitation (ChIP-on-Chip) and Affymetrix-based expression profiling to gain insight into how KLF11, a human transcription factor involved in tumor suppression and metabolic diseases, works by coupling to three co-factor groups: the Sin3-histone deacetylase system, WD40-domain containing proteins, and the HP1-histone methyltransferase system. Our results reveal that KLF11 regulates distinct gene networks involved in metabolism and growth by using single or combinatorial coupling events.Conclusion: This study, the first of its type for any KLF protein, reveals that interactions with multiple chromatin systems are required for the full gene regulatory function of these proteins.

Original languageEnglish (US)
Article number10
JournalBMC Molecular Biology
Volume15
DOIs
StatePublished - May 25 2014

Keywords

  • Cellular growth
  • Gene expression profiling
  • Gene networks
  • Krüppel-like factor
  • Metabolism
  • Proliferation
  • Signaling pathways
  • Transcription factor

ASJC Scopus subject areas

  • Molecular Biology

Fingerprint Dive into the research topics of 'Single and combinatorial chromatin coupling events underlies the function of transcript factor krüppel-like factor 11 in the regulation of gene networks'. Together they form a unique fingerprint.

  • Cite this