Simultaneous measurement of estrogen and progesterone receptors in tumor cytosols with use of 125 I-labeled estradiol and of 3H-R5020

S. N. Thibodeau, L. Freeman, N. S. Jiang

Research output: Contribution to journalArticle

32 Scopus citations

Abstract

We describe a dual-isotope assay for measuring the concentration of estrogen receptors and progesterone receptors in tumor cytosols. The concentrations of these receptors are derived from separate Scatchard-plot analyses of a single dextran-coated charcoal assay that incorporates both radioiodinated estradiol and tritiated R5020 as the labeled ligands. The two isotopes, radoiodine and tritium, are easily measured with a liquid-scintillation counter. The concentrations of estrogen and progesterone receptors derived from the dual-label assay are identical to values derived from the respective single-label assays. Moreover, values obtained from an assay with dextran-coated charcoal that incorporates radoiodinated estradiol are identical to those obtained from an assay in which tritiated estradiol is used as the labeled ligand. The dual-isotope assay requires both less time and less tissue.

Original languageEnglish (US)
Pages (from-to)687-691
Number of pages5
JournalClinical chemistry
Volume27
Issue number5
StatePublished - Jan 1 1981

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

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