Simultaneous immunoelectron microscopic visualization of protein B23 and C23 distribution in the HeLa cell nucleolus

M. Biggiogera, S. Fakan, S. H. Kaufmann, A. Black, J. H. Shaper, H. Busch

Research output: Contribution to journalArticle

85 Scopus citations

Abstract

The intranucleolar distribution of phosphoproteins B23 and C23 was visualized simultaneously by post-embedding immunoelectron microscopy in HeLa cell nucleoli, using specific antibodies. The data show that proteins B23 and C23 co-localize to the same nucleolar compartments, i.e., the dense fibrillar components and the granular component. Neither of the two antibodies is significantly associated with the fibrillar centers in these cells, although the fibrillar centers appear positive after silver staining. These findings suggest that other unidentified components must be responsible for the silver staining observed in the fibrillar centers of interphase nucleoli. The results are discussed in the light of previously reported data obtained by preembedding immunolabeling techniques and by silver staining, which both suggested a localization of protein C23 inside the fibrillar centers.

Original languageEnglish (US)
Pages (from-to)1371-1374
Number of pages4
JournalJournal of Histochemistry and Cytochemistry
Volume37
Issue number9
DOIs
StatePublished - Jan 1 1989

ASJC Scopus subject areas

  • Anatomy
  • Histology

Fingerprint Dive into the research topics of 'Simultaneous immunoelectron microscopic visualization of protein B23 and C23 distribution in the HeLa cell nucleolus'. Together they form a unique fingerprint.

Cite this