Simultaneous determination of d- and l-thyroxine in human serum by liquid chromatography with electrochemical detection

Ian D. Hay, Thomas M. Annesley, Nai S. Jiang, Colum A. Gorman

Research output: Contribution to journalArticle

37 Scopus citations

Abstract

A method for the determination of d- and l-thyroxine in human serum is described. The method involves extraction of thyroxine from serum and the separation of thyroxine enantiomers on a reversed-phase, high-performance liquid chromatographic column by use of a chiral eluent containing l-proline and cupric sulfate. Satisfactory resolution of the enantiomers of thyroxine, triiodothyronine, and reverse triiodothyronine can be achieved in 12 min and, employing amperometric detection to monitor the separation, the detection limit for serum thyroxine is in the range of 1-3 ng per injected sample.

Original languageEnglish (US)
Pages (from-to)383-390
Number of pages8
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume226
Issue number2
DOIs
StatePublished - Dec 11 1981

ASJC Scopus subject areas

  • Chemistry(all)

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