TY - JOUR
T1 - Simultaneous determination of d- and l-thyroxine in human serum by liquid chromatography with electrochemical detection
AU - Hay, Ian D.
AU - Annesley, Thomas M.
AU - Jiang, Nai S.
AU - Gorman, Colum A.
N1 - Funding Information:
This work was supported in part by a grant from Travenol Laboratories Inc., Deerfield, IJL, U_S_A_ I_D_H_ and T&LA. were fellows of the Mayo Graduate -School of Medicine. Part of the work was presented (by 1-D-H.) at the First International Symposium on the NeurochemicaI and Clinical Applications of LCEC, Indianapolis, IN, May, 1980. The authors thank Mr. D. Machacek for his highly qualified technical assistance_
PY - 1981/12/11
Y1 - 1981/12/11
N2 - A method for the determination of d- and l-thyroxine in human serum is described. The method involves extraction of thyroxine from serum and the separation of thyroxine enantiomers on a reversed-phase, high-performance liquid chromatographic column by use of a chiral eluent containing l-proline and cupric sulfate. Satisfactory resolution of the enantiomers of thyroxine, triiodothyronine, and reverse triiodothyronine can be achieved in 12 min and, employing amperometric detection to monitor the separation, the detection limit for serum thyroxine is in the range of 1-3 ng per injected sample.
AB - A method for the determination of d- and l-thyroxine in human serum is described. The method involves extraction of thyroxine from serum and the separation of thyroxine enantiomers on a reversed-phase, high-performance liquid chromatographic column by use of a chiral eluent containing l-proline and cupric sulfate. Satisfactory resolution of the enantiomers of thyroxine, triiodothyronine, and reverse triiodothyronine can be achieved in 12 min and, employing amperometric detection to monitor the separation, the detection limit for serum thyroxine is in the range of 1-3 ng per injected sample.
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U2 - 10.1016/S0378-4347(00)86072-7
DO - 10.1016/S0378-4347(00)86072-7
M3 - Article
C2 - 7320167
AN - SCOPUS:0019853753
SN - 0378-4347
VL - 226
SP - 383
EP - 390
JO - Journal of Chromatography B: Biomedical Sciences and Applications
JF - Journal of Chromatography B: Biomedical Sciences and Applications
IS - 2
ER -