Simple and sure methodology for massive hepatectomy in the mouse

Tomohide Hori, Norifumi Ohashi, Feng Chen, Ann Marie T. Baine, Lindsay B. Gardner, Sura Jermanus, Justin H. Nguyen

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Background Reliable models for massive hepatectomy in the mouse are required for experimental liver research. Methods We analyzed anatomical findings in 100 mice following massive hepatectomy induced by liver reduction >70%. The impact of various factors in the different models was also analyzed, including learning curves, operative time, survival curves and histopathological findings. Results According to anatomical results, murine models with 75%, 80% and 90% of liver resection produced massive hepatectomy. Learning curves and operative times were most optimal with the clip technique. Each hepatectomy performed using the clip technique produced a reasonable survival curve, and there were no differences in histopathological findings between the suture and clip techniques. Conclusion Massive hepatectomy by the clip technique is simple and can provide reliable and relevant data.

Original languageEnglish (US)
Pages (from-to)307-318
Number of pages12
JournalAnnals of Gastroenterology
Volume24
Issue number4
StatePublished - Nov 21 2011

Keywords

  • Animal model
  • Clip
  • Hepatectomy
  • Microsurgery
  • Mouse
  • Surgical technique

ASJC Scopus subject areas

  • Gastroenterology

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    Hori, T., Ohashi, N., Chen, F., Baine, A. M. T., Gardner, L. B., Jermanus, S., & Nguyen, J. H. (2011). Simple and sure methodology for massive hepatectomy in the mouse. Annals of Gastroenterology, 24(4), 307-318.