Similar responsiveness of smooth muscle of the canine basilar artery to EDRF and nitric oxide

Zvonimir S Katusic, J. J. Marshall, H. A. Kontos, P. M. Vanhoutte

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Experiments were designed to compare the reactivity of canine basilar arteries to endothelium-derived relaxing factor (EDRF) and nitric oxide. Preparations with endothelium activated by bradykinin were used to study relaxations induced with EDRF, whereas the inhibitory effect of nitric oxide was studied in preparations without endothelium. All experiments were performed in the presence of indomethacin. EDRF- and nitric oxide-induced relaxations were significantly augmented in the presence of superoxide dismutase plus catalase but were reduced in the presence of methylene blue, LY 83583, and hemoglobin. M and B 22984 did not affect relaxations to either EDRF or nitric oxide. These results indicate that in the canine basilar artery EDRF is not an oxygen-derived free radical. The similar responsiveness of the basilar artery to EDRF and nitric oxide is consistent with the proposal that in the canine basilar artery nitric oxide is the factor.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume257
Issue number4
StatePublished - 1989

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Endothelium-Dependent Relaxing Factors
Basilar Artery
Smooth Muscle
Canidae
Nitric Oxide
Endothelium
6-anilino-5,8-quinolinedione
Methylene Blue
Bradykinin
Indomethacin
Catalase
Superoxide Dismutase
Free Radicals
Hemoglobins
Oxygen

ASJC Scopus subject areas

  • Physiology

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Similar responsiveness of smooth muscle of the canine basilar artery to EDRF and nitric oxide. / Katusic, Zvonimir S; Marshall, J. J.; Kontos, H. A.; Vanhoutte, P. M.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 257, No. 4, 1989.

Research output: Contribution to journalArticle

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