Side chain oxidation of 25-hydroxy-[26,27-14C]vitamin D3 and 1,25-dihydroxy-[26,27-14C]vitamin D3 in vivo by chickens

Rajiv Kumar; H. F. DeLuca

doi:10.1016/S0006-291X(76)80291-4

Side chain oxidation of 25-hydroxy-[26,27-¹⁴C]vitamin D₃ and 1,25-dihydroxy-[26,27-¹⁴C]vitamin D₃ in vivo by chickens

Rajiv Kumar, H. F. DeLuca

Nephrology and Hypertension

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Radio carbon dioxide was detected in expired air of vitamin D-deficient chicks administered either 25-hydroxy-[26,27-¹⁴C]vitamin D₃ or 1,25-dihydroxy-[26,27-¹⁴C]vitamin D₃ intravenously. At 48 hours the amounts were 5.13 ± 1.02% and 11.8 ± 3.10% of the administered dose, respectively. This phenomenon, similar to the one observed in rats, represents side chain oxidation and elimination of either one or both of the 26 and 27 carbons from the viamin D molecule. The observation that the rate of ¹⁴CO₂ evolution is maximal 4 hours after a dose of 1,25-dihydroxy-[26,27-¹⁴C]vitamin D₃ suggests that this pathway may be of importance to the function of 1,25-dihydroxyvitamin D₃.

Original language	English (US)
Pages (from-to)	197-200
Number of pages	4
Journal	Biochemical and Biophysical Research Communications
Volume	69
Issue number	1
DOIs	https://doi.org/10.1016/S0006-291X(76)80291-4
State	Published - Mar 8 1976

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "Side chain oxidation of 25-hydroxy-[26,27-14C]vitamin D3 and 1,25-dihydroxy-[26,27-14C]vitamin D3 in vivo by chickens",

abstract = "Radio carbon dioxide was detected in expired air of vitamin D-deficient chicks administered either 25-hydroxy-[26,27-14C]vitamin D3 or 1,25-dihydroxy-[26,27-14C]vitamin D3 intravenously. At 48 hours the amounts were 5.13 ± 1.02% and 11.8 ± 3.10% of the administered dose, respectively. This phenomenon, similar to the one observed in rats, represents side chain oxidation and elimination of either one or both of the 26 and 27 carbons from the viamin D molecule. The observation that the rate of 14CO2 evolution is maximal 4 hours after a dose of 1,25-dihydroxy-[26,27-14C]vitamin D3 suggests that this pathway may be of importance to the function of 1,25-dihydroxyvitamin D3.",

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AU - DeLuca, H. F.

PY - 1976/3/8

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N2 - Radio carbon dioxide was detected in expired air of vitamin D-deficient chicks administered either 25-hydroxy-[26,27-14C]vitamin D3 or 1,25-dihydroxy-[26,27-14C]vitamin D3 intravenously. At 48 hours the amounts were 5.13 ± 1.02% and 11.8 ± 3.10% of the administered dose, respectively. This phenomenon, similar to the one observed in rats, represents side chain oxidation and elimination of either one or both of the 26 and 27 carbons from the viamin D molecule. The observation that the rate of 14CO2 evolution is maximal 4 hours after a dose of 1,25-dihydroxy-[26,27-14C]vitamin D3 suggests that this pathway may be of importance to the function of 1,25-dihydroxyvitamin D3.

AB - Radio carbon dioxide was detected in expired air of vitamin D-deficient chicks administered either 25-hydroxy-[26,27-14C]vitamin D3 or 1,25-dihydroxy-[26,27-14C]vitamin D3 intravenously. At 48 hours the amounts were 5.13 ± 1.02% and 11.8 ± 3.10% of the administered dose, respectively. This phenomenon, similar to the one observed in rats, represents side chain oxidation and elimination of either one or both of the 26 and 27 carbons from the viamin D molecule. The observation that the rate of 14CO2 evolution is maximal 4 hours after a dose of 1,25-dihydroxy-[26,27-14C]vitamin D3 suggests that this pathway may be of importance to the function of 1,25-dihydroxyvitamin D3.

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Side chain oxidation of 25-hydroxy-[26,27-¹⁴C]vitamin D₃ and 1,25-dihydroxy-[26,27-¹⁴C]vitamin D₃ in vivo by chickens

Abstract

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