Side chain oxidation of 25-hydroxy-[26,27-14C]vitamin D3 and 1,25-dihydroxy-[26,27-14C]vitamin D3 in vivo by chickens

Rajiv Kumar, H. F. DeLuca

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Radio carbon dioxide was detected in expired air of vitamin D-deficient chicks administered either 25-hydroxy-[26,27-14C]vitamin D3 or 1,25-dihydroxy-[26,27-14C]vitamin D3 intravenously. At 48 hours the amounts were 5.13 ± 1.02% and 11.8 ± 3.10% of the administered dose, respectively. This phenomenon, similar to the one observed in rats, represents side chain oxidation and elimination of either one or both of the 26 and 27 carbons from the viamin D molecule. The observation that the rate of 14CO2 evolution is maximal 4 hours after a dose of 1,25-dihydroxy-[26,27-14C]vitamin D3 suggests that this pathway may be of importance to the function of 1,25-dihydroxyvitamin D3.

Original languageEnglish (US)
Pages (from-to)197-200
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume69
Issue number1
DOIs
StatePublished - Mar 8 1976
Externally publishedYes

Fingerprint

Cholecalciferol
Chickens
Oxidation
Calcitriol
Radio
Carbon Dioxide
Vitamin D
Rats
Carbon
Air
Molecules

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

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title = "Side chain oxidation of 25-hydroxy-[26,27-14C]vitamin D3 and 1,25-dihydroxy-[26,27-14C]vitamin D3 in vivo by chickens",
abstract = "Radio carbon dioxide was detected in expired air of vitamin D-deficient chicks administered either 25-hydroxy-[26,27-14C]vitamin D3 or 1,25-dihydroxy-[26,27-14C]vitamin D3 intravenously. At 48 hours the amounts were 5.13 ± 1.02{\%} and 11.8 ± 3.10{\%} of the administered dose, respectively. This phenomenon, similar to the one observed in rats, represents side chain oxidation and elimination of either one or both of the 26 and 27 carbons from the viamin D molecule. The observation that the rate of 14CO2 evolution is maximal 4 hours after a dose of 1,25-dihydroxy-[26,27-14C]vitamin D3 suggests that this pathway may be of importance to the function of 1,25-dihydroxyvitamin D3.",
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T1 - Side chain oxidation of 25-hydroxy-[26,27-14C]vitamin D3 and 1,25-dihydroxy-[26,27-14C]vitamin D3 in vivo by chickens

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AB - Radio carbon dioxide was detected in expired air of vitamin D-deficient chicks administered either 25-hydroxy-[26,27-14C]vitamin D3 or 1,25-dihydroxy-[26,27-14C]vitamin D3 intravenously. At 48 hours the amounts were 5.13 ± 1.02% and 11.8 ± 3.10% of the administered dose, respectively. This phenomenon, similar to the one observed in rats, represents side chain oxidation and elimination of either one or both of the 26 and 27 carbons from the viamin D molecule. The observation that the rate of 14CO2 evolution is maximal 4 hours after a dose of 1,25-dihydroxy-[26,27-14C]vitamin D3 suggests that this pathway may be of importance to the function of 1,25-dihydroxyvitamin D3.

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