Side chain metabolism of 25 hydroxy [26,27 14C]vitamin D3 and 1,25 dihydroxy [26,27 14C]vitamin D3 in vivo

D. Harnden, Rajiv Kumar, M. F. Holick, H. F. DeLuca

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Abstract

Radioactive CO2 was detected in expired air after the administration of 25 hydroxy [26,27 14C]vitamin D3 to vitamin D deficient hypocalcemic rats; 14CO2 was also detected after the administration of 1,25 dihydroxy [26,27 14C]vitamin D3 to rats raised on the same diet. Nephrectomy totally abolished 14CO2 formation after administration of 25 hydroxy [26,27 14C]vitamin D3, but not after the administration of 1,25 dihydroxy [26,27 14C]vitamin D3. The production of 14CO2 commenced within 4 hours after injection of 1,25 dihydroxy [26,27 14C]vitamin D3, suggesting a possible relevance of this reaction to the function of 1,25 dihydroxyvitamin D3. These results at least demonstrate a new metabolic pathway of vitamin D3 metabolism involving the oxidation of a portion of the side chain of 1,25 dihydroxyvitamin D3 to CO2.

Original languageEnglish (US)
Pages (from-to)493-494
Number of pages2
JournalScience
Volume193
Issue number4252
StatePublished - 1976
Externally publishedYes

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