TY - JOUR
T1 - Side chain metabolism of 25-hydroxy-[26,27-14C]vitamin D 3 and 1,25-dihydroxy-[26,27-14C]vitamin D3 in vivo
AU - Harnden, Deborah
AU - Kumar, Rajiv
AU - Holick, M. F.
AU - DeLuca, H. F.
PY - 1976
Y1 - 1976
N2 - Radioactive CO2 was detected in expired air after the administration of 25-hydroxy-[26,27-14C]vitamin D3 to vitamin D-deficient hypocalcemic rats; 14CO2 was also detected after the administration of 1,25-dihydroxy-[26,27-14C] vitamin D3 to rats raised on the same diet. Nephrectomy totally abolished 14CO2 formation after administration of 25-hydroxy-[26,27-14C]vitamin D3, but not after the administration of 1,25-dihydroxy-[26,27-14C]vitamin D3. The production of 14CO2 commenced within 4 hours after injection of 1,25-dihydroxy-[26,27-14C]vitamin D3, suggesting a possible relevance of this reaction to the function of 1,25-dihydroxyvitamin D3. These results at least demonstrate a new metabolic pathway of vitamin D3 metabolism involving the oxidation of a portion of the side chain of 1,25-dihydroxyvitamin D3 to CO 2.
AB - Radioactive CO2 was detected in expired air after the administration of 25-hydroxy-[26,27-14C]vitamin D3 to vitamin D-deficient hypocalcemic rats; 14CO2 was also detected after the administration of 1,25-dihydroxy-[26,27-14C] vitamin D3 to rats raised on the same diet. Nephrectomy totally abolished 14CO2 formation after administration of 25-hydroxy-[26,27-14C]vitamin D3, but not after the administration of 1,25-dihydroxy-[26,27-14C]vitamin D3. The production of 14CO2 commenced within 4 hours after injection of 1,25-dihydroxy-[26,27-14C]vitamin D3, suggesting a possible relevance of this reaction to the function of 1,25-dihydroxyvitamin D3. These results at least demonstrate a new metabolic pathway of vitamin D3 metabolism involving the oxidation of a portion of the side chain of 1,25-dihydroxyvitamin D3 to CO 2.
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U2 - 10.1126/science.941020
DO - 10.1126/science.941020
M3 - Article
C2 - 941020
AN - SCOPUS:0017136897
SN - 0036-8075
VL - 193
SP - 493
EP - 494
JO - Science
JF - Science
IS - 4252
ER -