Side chain metabolism of 25-hydroxy-[26,27-14C]vitamin D 3 and 1,25-dihydroxy-[26,27-14C]vitamin D3 in vivo

Deborah Harnden, Rajiv Kumar, M. F. Holick, H. F. DeLuca

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41 Scopus citations


Radioactive CO2 was detected in expired air after the administration of 25-hydroxy-[26,27-14C]vitamin D3 to vitamin D-deficient hypocalcemic rats; 14CO2 was also detected after the administration of 1,25-dihydroxy-[26,27-14C] vitamin D3 to rats raised on the same diet. Nephrectomy totally abolished 14CO2 formation after administration of 25-hydroxy-[26,27-14C]vitamin D3, but not after the administration of 1,25-dihydroxy-[26,27-14C]vitamin D3. The production of 14CO2 commenced within 4 hours after injection of 1,25-dihydroxy-[26,27-14C]vitamin D3, suggesting a possible relevance of this reaction to the function of 1,25-dihydroxyvitamin D3. These results at least demonstrate a new metabolic pathway of vitamin D3 metabolism involving the oxidation of a portion of the side chain of 1,25-dihydroxyvitamin D3 to CO 2.

Original languageEnglish (US)
Pages (from-to)493-494
Number of pages2
Issue number4252
StatePublished - 1976

ASJC Scopus subject areas

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