Shrinkage of pegylated and non-pegylated liposomes in serum

Joy Wolfram; Krishna Suri; Yong Yang; Jianliang Shen; Christian Celia; Massimo Fresta; Yuliang Zhao; Haifa Shen; Mauro Ferrari

doi:10.1016/j.colsurfb.2013.10.009

Shrinkage of pegylated and non-pegylated liposomes in serum

Joy Wolfram, Krishna Suri, Yong Yang, Jianliang Shen, Christian Celia, Massimo Fresta, Yuliang Zhao, Haifa Shen, Mauro Ferrari

Biochemistry and Molecular Biology

Research output: Contribution to journal › Article › peer-review

78 Scopus citations

Abstract

An essential requisite for the design of nanodelivery systems is the ability to characterize the size, homogeneity and zeta potential of nanoparticles. Such properties can be tailored in order to create the most efficient drug delivery platforms. An important question is whether these characteristics change upon systemic injection. Here, we have studied the behavior of phosphatidylcholine/cholesterol liposomes exposed to serum proteins. The results reveal a serum-induced reduction in the size and homogeneity of both pegylated and non-pegylated liposomes, implicating the possible role of osmotic forces. In addition, changes to zeta-potential were observed upon exposing liposomes to serum. The liposomes with polyethylene glycol expressed different characteristics than their non-polymeric counterparts, suggesting the potential formation of a denser protein corona around the non-pegylated liposomes.

Original language	English (US)
Pages (from-to)	294-300
Number of pages	7
Journal	Colloids and Surfaces B: Biointerfaces
Volume	114
DOIs	https://doi.org/10.1016/j.colsurfb.2013.10.009
State	Published - Feb 1 2014

Keywords

Dynamic light scattering
Liposomes
Polydispersity index
Serum
Size
Zeta potential

ASJC Scopus subject areas

Biotechnology
Surfaces and Interfaces
Physical and Theoretical Chemistry
Colloid and Surface Chemistry

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10.1016/j.colsurfb.2013.10.009

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title = "Shrinkage of pegylated and non-pegylated liposomes in serum",

abstract = "An essential requisite for the design of nanodelivery systems is the ability to characterize the size, homogeneity and zeta potential of nanoparticles. Such properties can be tailored in order to create the most efficient drug delivery platforms. An important question is whether these characteristics change upon systemic injection. Here, we have studied the behavior of phosphatidylcholine/cholesterol liposomes exposed to serum proteins. The results reveal a serum-induced reduction in the size and homogeneity of both pegylated and non-pegylated liposomes, implicating the possible role of osmotic forces. In addition, changes to zeta-potential were observed upon exposing liposomes to serum. The liposomes with polyethylene glycol expressed different characteristics than their non-polymeric counterparts, suggesting the potential formation of a denser protein corona around the non-pegylated liposomes.",

keywords = "Dynamic light scattering, Liposomes, Polydispersity index, Serum, Size, Zeta potential",

author = "Joy Wolfram and Krishna Suri and Yong Yang and Jianliang Shen and Christian Celia and Massimo Fresta and Yuliang Zhao and Haifa Shen and Mauro Ferrari",

note = "Funding Information: The research was supported by funds from the Methodist Hospital Research Institute . Partial funds were acquired from: the Ernest Cockrell Jr. Distinguished Endowed Chair (M.F.), the US Department of Defense ( W81XWH-09-1-0212 ) (M.F.), the National Institute of Health ( U54CA143837 , U54CA151668 ) (M.F.), Nylands nation Finland (J.W.), Department of Defense grant W81XWH-12-1-0414 (M.F.) and the State of Texas CPRIT grant RP121071 (M.F. and H.S.). We thank Matthew Landry for assistance with the figures.",

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AU - Wolfram, Joy

AU - Suri, Krishna

AU - Yang, Yong

AU - Shen, Jianliang

AU - Celia, Christian

AU - Fresta, Massimo

AU - Zhao, Yuliang

AU - Shen, Haifa

AU - Ferrari, Mauro

N1 - Funding Information: The research was supported by funds from the Methodist Hospital Research Institute . Partial funds were acquired from: the Ernest Cockrell Jr. Distinguished Endowed Chair (M.F.), the US Department of Defense ( W81XWH-09-1-0212 ) (M.F.), the National Institute of Health ( U54CA143837 , U54CA151668 ) (M.F.), Nylands nation Finland (J.W.), Department of Defense grant W81XWH-12-1-0414 (M.F.) and the State of Texas CPRIT grant RP121071 (M.F. and H.S.). We thank Matthew Landry for assistance with the figures.

PY - 2014/2/1

Y1 - 2014/2/1

N2 - An essential requisite for the design of nanodelivery systems is the ability to characterize the size, homogeneity and zeta potential of nanoparticles. Such properties can be tailored in order to create the most efficient drug delivery platforms. An important question is whether these characteristics change upon systemic injection. Here, we have studied the behavior of phosphatidylcholine/cholesterol liposomes exposed to serum proteins. The results reveal a serum-induced reduction in the size and homogeneity of both pegylated and non-pegylated liposomes, implicating the possible role of osmotic forces. In addition, changes to zeta-potential were observed upon exposing liposomes to serum. The liposomes with polyethylene glycol expressed different characteristics than their non-polymeric counterparts, suggesting the potential formation of a denser protein corona around the non-pegylated liposomes.

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KW - Dynamic light scattering

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KW - Polydispersity index

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KW - Size

KW - Zeta potential

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Shrinkage of pegylated and non-pegylated liposomes in serum

Abstract

Keywords

ASJC Scopus subject areas

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