Short-term immunosuppression and surgical neoangionenesis with host vessels maintains long-term viability of vascularized bone allografts

Michael Pelzer, Mikko Larsen, Yang Guk Chung, Teruyasu Ohno, Jeffrey L. Platt, Patricia F. Friedrich, Allen T. Bishop

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

Currently available methods to reconstruct large skeletal defects have limitations. These include nonunion and stress fractures in structural allografts, and inability to match the size, shape, and/or strength of most recipient sites using vascularized fibular autografts. Prosthetic diaphyseal replacements may loosen or produce periprosthetic fractures. Transplantation of living allogenic bone would enable matching donor bone to the recipient site, combined with the desirable healing and remodeling properties of living bone. We propose a novel method by which the transplantation of such tissue might be done without the risks of life-long immunosuppression, using surgical neoangiogenesis to develop a new host-derived osseous blood supply. We performed vascularized femoral allografts from 86 female Dark Agouti donor rats to male Piebald Virol Glaxo recipients across a major histocompatibility (MHC) barrier. In addition to microvascular reconstruction of the nutrient vessel, we surgically implanted a host arteriovenous (AV) bundle into the medullary canal to promote host vessel neoangiogenesis. Independent variables included patency of the implanted AV bundle, and use of 2 weeks' FK-506 immunosuppression. After 18 weeks, bone blood flow was measured, and neoangiogenic capillary density quantified. Bone blood flow and capillary density were significantly greater in transiently immunosuppressed recipients with a patent AV pedicle. We conclude that neoangiogenesis from implanted host-derived AV-bundles, combined with short-term immunosuppression maintains blood flow in vascularized bone allografts, and offers potential for clinical application.

Original languageEnglish (US)
Pages (from-to)370-377
Number of pages8
JournalJournal of Orthopaedic Research
Volume25
Issue number3
DOIs
StatePublished - Mar 1 2007

Keywords

  • Host vessels
  • Short-term immunosuppression
  • Vascularized bone allografts

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

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