TY - JOUR
T1 - Shifting the optimal stiffness for cell migration
AU - Bangasser, Benjamin L.
AU - Shamsan, Ghaidan A.
AU - Chan, Clarence E.
AU - Opoku, Kwaku N.
AU - Tüzel, Erkan
AU - Schlichtmann, Benjamin W.
AU - Kasim, Jesse A.
AU - Fuller, Benjamin J.
AU - McCullough, Brannon R.
AU - Rosenfeld, Steven S.
AU - Odde, David J.
N1 - Publisher Copyright:
© The Author(s) 2017.
PY - 2017/5/22
Y1 - 2017/5/22
N2 - Cell migration, which is central to many biological processes including wound healing and cancer progression, is sensitive to environmental stiffness, and many cell types exhibit a stiffness optimum, at which migration is maximal. Here we present a cell migration simulator that predicts a stiffness optimum that can be shifted by altering the number of active molecular motors and clutches. This prediction is verified experimentally by comparing cell traction and F-actin retrograde flow for two cell types with differing amounts of active motors and clutches: embryonic chick forebrain neurons (ECFNs; optimum ∼ 1 kPa) and U251 glioma cells (optimum ∼ 100 kPa). In addition, the model predicts, and experiments confirm, that the stiffness optimum of U251 glioma cell migration, morphology and F-actin retrograde flow rate can be shifted to lower stiffness by simultaneous drug inhibition of myosin II motors and integrin-mediated adhesions.
AB - Cell migration, which is central to many biological processes including wound healing and cancer progression, is sensitive to environmental stiffness, and many cell types exhibit a stiffness optimum, at which migration is maximal. Here we present a cell migration simulator that predicts a stiffness optimum that can be shifted by altering the number of active molecular motors and clutches. This prediction is verified experimentally by comparing cell traction and F-actin retrograde flow for two cell types with differing amounts of active motors and clutches: embryonic chick forebrain neurons (ECFNs; optimum ∼ 1 kPa) and U251 glioma cells (optimum ∼ 100 kPa). In addition, the model predicts, and experiments confirm, that the stiffness optimum of U251 glioma cell migration, morphology and F-actin retrograde flow rate can be shifted to lower stiffness by simultaneous drug inhibition of myosin II motors and integrin-mediated adhesions.
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U2 - 10.1038/ncomms15313
DO - 10.1038/ncomms15313
M3 - Article
C2 - 28530245
AN - SCOPUS:85020286424
SN - 2041-1723
VL - 8
JO - Nature communications
JF - Nature communications
M1 - 15313
ER -