Sharpening spots: Correcting for bleedover in cDNA array images

Terry Therneau; Renee C. Tschumper; Diane Jelinek

doi:10.1016/S0025-5564(01)00101-8

Sharpening spots: Correcting for bleedover in cDNA array images

Terry Therneau, Renee C. Tschumper, Diane Jelinek

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

For cDNA array methods that depend on imaging of a radiolabel, we show that bleedover of one spot onto another, due to the gap between the array and the imaging media, can be a major problem. The images can be sharpened, however, using a blind convolution method based on the EM algorithm. The sharpened images look like a set of donuts, which concurs with our knowledge of the spotting process. Oversharpened images are actually useful as well, in locating the centers of each spot.

Original language	English (US)
Pages (from-to)	1-15
Number of pages	15
Journal	Mathematical Biosciences
Volume	176
Issue number	1
DOIs	https://doi.org/10.1016/S0025-5564(01)00101-8
State	Published - 2002

Keywords

EM algorithm
Fast Fourier transform
Image processing
cDNA arrays

ASJC Scopus subject areas

Statistics and Probability
Modeling and Simulation
Biochemistry, Genetics and Molecular Biology(all)
Immunology and Microbiology(all)
Agricultural and Biological Sciences(all)
Applied Mathematics

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10.1016/S0025-5564(01)00101-8

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abstract = "For cDNA array methods that depend on imaging of a radiolabel, we show that bleedover of one spot onto another, due to the gap between the array and the imaging media, can be a major problem. The images can be sharpened, however, using a blind convolution method based on the EM algorithm. The sharpened images look like a set of donuts, which concurs with our knowledge of the spotting process. Oversharpened images are actually useful as well, in locating the centers of each spot.",

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AU - Jelinek, Diane

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AB - For cDNA array methods that depend on imaging of a radiolabel, we show that bleedover of one spot onto another, due to the gap between the array and the imaging media, can be a major problem. The images can be sharpened, however, using a blind convolution method based on the EM algorithm. The sharpened images look like a set of donuts, which concurs with our knowledge of the spotting process. Oversharpened images are actually useful as well, in locating the centers of each spot.

KW - EM algorithm

KW - Fast Fourier transform

KW - Image processing

KW - cDNA arrays

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Sharpening spots: Correcting for bleedover in cDNA array images

Abstract

Keywords

ASJC Scopus subject areas

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