Sex steroids modulate prolactin action in spontaneously luteinizing porcine granulosa cells in vitro

Johannes D Veldhuis, P. A. Klase, J. M. Hammond

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Prolactin (20-1000 ng/ml) stimulated progesterone secretion in monolayer cultures of granulosa cells isolated from mature (>8 mm) porcine follicles. The action of 17β-estradiol alone and the interaction between estradiol and prolactin in controlling steroidogenesis were critically dependent upon the duration of hormone treatment. Acute administration (within 36 h) of 17β-estradiol, but not 17α-estradiol, suppressed both spontaneous and prolactin-stimulated progesterone production. Continuation of estrogen treatment beyond 48 h markedly increased progesterone secretion per cell and with prolactin produced synergistic enhancement of progesterone accumulation. However, 5α-dihydrotestosterone also significantly augmented progesterone production after both acute and prolonged administration. The steroidogenic effect of prolactin was greater in the presence of 5α-dihydrotestosterone, but did not elicit true synergism. Measurements of sex-steroids levels in porcine follicular fluids indicated that estrogen concentrations that were biologically active in vitro are similar to those attained in vivo. These observations suggest a major role for sex-steroid hormones both in the control of basal steroidogenesis and in the modulation of prolactin action in mature spontaneously luteinizing granulosa cells.

Original languageEnglish (US)
Pages (from-to)1463-1468
Number of pages6
JournalEndocrinology
Volume108
Issue number4
StatePublished - 1981
Externally publishedYes

Fingerprint

Granulosa Cells
Prolactin
Swine
Steroids
Progesterone
Estradiol
Dihydrotestosterone
Estrogens
Follicular Fluid
Gonadal Steroid Hormones
In Vitro Techniques
Hormones

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Veldhuis, J. D., Klase, P. A., & Hammond, J. M. (1981). Sex steroids modulate prolactin action in spontaneously luteinizing porcine granulosa cells in vitro. Endocrinology, 108(4), 1463-1468.

Sex steroids modulate prolactin action in spontaneously luteinizing porcine granulosa cells in vitro. / Veldhuis, Johannes D; Klase, P. A.; Hammond, J. M.

In: Endocrinology, Vol. 108, No. 4, 1981, p. 1463-1468.

Research output: Contribution to journalArticle

Veldhuis, JD, Klase, PA & Hammond, JM 1981, 'Sex steroids modulate prolactin action in spontaneously luteinizing porcine granulosa cells in vitro', Endocrinology, vol. 108, no. 4, pp. 1463-1468.
Veldhuis, Johannes D ; Klase, P. A. ; Hammond, J. M. / Sex steroids modulate prolactin action in spontaneously luteinizing porcine granulosa cells in vitro. In: Endocrinology. 1981 ; Vol. 108, No. 4. pp. 1463-1468.
@article{7e1f260ff88c4d92990bad589c795706,
title = "Sex steroids modulate prolactin action in spontaneously luteinizing porcine granulosa cells in vitro",
abstract = "Prolactin (20-1000 ng/ml) stimulated progesterone secretion in monolayer cultures of granulosa cells isolated from mature (>8 mm) porcine follicles. The action of 17β-estradiol alone and the interaction between estradiol and prolactin in controlling steroidogenesis were critically dependent upon the duration of hormone treatment. Acute administration (within 36 h) of 17β-estradiol, but not 17α-estradiol, suppressed both spontaneous and prolactin-stimulated progesterone production. Continuation of estrogen treatment beyond 48 h markedly increased progesterone secretion per cell and with prolactin produced synergistic enhancement of progesterone accumulation. However, 5α-dihydrotestosterone also significantly augmented progesterone production after both acute and prolonged administration. The steroidogenic effect of prolactin was greater in the presence of 5α-dihydrotestosterone, but did not elicit true synergism. Measurements of sex-steroids levels in porcine follicular fluids indicated that estrogen concentrations that were biologically active in vitro are similar to those attained in vivo. These observations suggest a major role for sex-steroid hormones both in the control of basal steroidogenesis and in the modulation of prolactin action in mature spontaneously luteinizing granulosa cells.",
author = "Veldhuis, {Johannes D} and Klase, {P. A.} and Hammond, {J. M.}",
year = "1981",
language = "English (US)",
volume = "108",
pages = "1463--1468",
journal = "Endocrinology",
issn = "0013-7227",
publisher = "The Endocrine Society",
number = "4",

}

TY - JOUR

T1 - Sex steroids modulate prolactin action in spontaneously luteinizing porcine granulosa cells in vitro

AU - Veldhuis, Johannes D

AU - Klase, P. A.

AU - Hammond, J. M.

PY - 1981

Y1 - 1981

N2 - Prolactin (20-1000 ng/ml) stimulated progesterone secretion in monolayer cultures of granulosa cells isolated from mature (>8 mm) porcine follicles. The action of 17β-estradiol alone and the interaction between estradiol and prolactin in controlling steroidogenesis were critically dependent upon the duration of hormone treatment. Acute administration (within 36 h) of 17β-estradiol, but not 17α-estradiol, suppressed both spontaneous and prolactin-stimulated progesterone production. Continuation of estrogen treatment beyond 48 h markedly increased progesterone secretion per cell and with prolactin produced synergistic enhancement of progesterone accumulation. However, 5α-dihydrotestosterone also significantly augmented progesterone production after both acute and prolonged administration. The steroidogenic effect of prolactin was greater in the presence of 5α-dihydrotestosterone, but did not elicit true synergism. Measurements of sex-steroids levels in porcine follicular fluids indicated that estrogen concentrations that were biologically active in vitro are similar to those attained in vivo. These observations suggest a major role for sex-steroid hormones both in the control of basal steroidogenesis and in the modulation of prolactin action in mature spontaneously luteinizing granulosa cells.

AB - Prolactin (20-1000 ng/ml) stimulated progesterone secretion in monolayer cultures of granulosa cells isolated from mature (>8 mm) porcine follicles. The action of 17β-estradiol alone and the interaction between estradiol and prolactin in controlling steroidogenesis were critically dependent upon the duration of hormone treatment. Acute administration (within 36 h) of 17β-estradiol, but not 17α-estradiol, suppressed both spontaneous and prolactin-stimulated progesterone production. Continuation of estrogen treatment beyond 48 h markedly increased progesterone secretion per cell and with prolactin produced synergistic enhancement of progesterone accumulation. However, 5α-dihydrotestosterone also significantly augmented progesterone production after both acute and prolonged administration. The steroidogenic effect of prolactin was greater in the presence of 5α-dihydrotestosterone, but did not elicit true synergism. Measurements of sex-steroids levels in porcine follicular fluids indicated that estrogen concentrations that were biologically active in vitro are similar to those attained in vivo. These observations suggest a major role for sex-steroid hormones both in the control of basal steroidogenesis and in the modulation of prolactin action in mature spontaneously luteinizing granulosa cells.

UR - http://www.scopus.com/inward/record.url?scp=0019451267&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019451267&partnerID=8YFLogxK

M3 - Article

C2 - 7472275

AN - SCOPUS:0019451267

VL - 108

SP - 1463

EP - 1468

JO - Endocrinology

JF - Endocrinology

SN - 0013-7227

IS - 4

ER -