Sex differences in translocator protein 18 kDa (TSPO) in the heart: Implications for imaging myocardial inflammation

DeLisa Fairweather, Michael J. Coronado, Amanda E. Garton, Jennifer L. Dziedzic, Adriana Bucek, Leslie T Jr. Cooper, Jessica E. Brandt, Fatima S. Alikhan, Haofan Wang, Christopher J. Endres, Judy Choi, Martin G. Pomper, Tomás R. Guilarte

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Myocarditis is more severe in men than in women and difficult to diagnose due to a lack of imaging modalities that directly detect myocardial inflammation. Translocator protein 18 kDa (TSPO) is used extensively to image brain inflammation due to its presence in CD11b+ brain microglia. In this study, we examined expression of TSPO and CD11b in mice with coxsackievirus B3 (CVB3) myocarditis and biopsy sections from myocarditis patients in order to determine if it could be used to image myocarditis. We found that male mice with CVB3 myocarditis upregulated more genes associated with TSPO activation than female mice. TSPO expression was increased in the heart of male mice and men with myocarditis compared with female subjects due to testosterone, where it was expressed predominantly in CD11b+ immune cells. We show that TSPO ligands detect myocardial inflammation using microSPECT, with increased uptake of [125I]-IodoDPA-713 in male mice with CVB3 myocarditis compared with undiseased controls.

Original languageEnglish (US)
Pages (from-to)192-202
Number of pages11
JournalJournal of Cardiovascular Translational Research
Volume7
Issue number2
DOIs
StatePublished - 2014

Fingerprint

Myocarditis
Sex Characteristics
Inflammation
Enterovirus
Proteins
Microglia
Encephalitis
Testosterone
Ligands
Biopsy
Brain
Genes

Keywords

  • CD11b
  • Imaging
  • Myocarditis
  • Sex differences
  • Single-photon emission computed tomography
  • TSPO

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Genetics
  • Genetics(clinical)
  • Molecular Medicine
  • Pharmaceutical Science

Cite this

Sex differences in translocator protein 18 kDa (TSPO) in the heart : Implications for imaging myocardial inflammation. / Fairweather, DeLisa; Coronado, Michael J.; Garton, Amanda E.; Dziedzic, Jennifer L.; Bucek, Adriana; Cooper, Leslie T Jr.; Brandt, Jessica E.; Alikhan, Fatima S.; Wang, Haofan; Endres, Christopher J.; Choi, Judy; Pomper, Martin G.; Guilarte, Tomás R.

In: Journal of Cardiovascular Translational Research, Vol. 7, No. 2, 2014, p. 192-202.

Research output: Contribution to journalArticle

Fairweather, D, Coronado, MJ, Garton, AE, Dziedzic, JL, Bucek, A, Cooper, LTJ, Brandt, JE, Alikhan, FS, Wang, H, Endres, CJ, Choi, J, Pomper, MG & Guilarte, TR 2014, 'Sex differences in translocator protein 18 kDa (TSPO) in the heart: Implications for imaging myocardial inflammation', Journal of Cardiovascular Translational Research, vol. 7, no. 2, pp. 192-202. https://doi.org/10.1007/s12265-013-9538-0
Fairweather, DeLisa ; Coronado, Michael J. ; Garton, Amanda E. ; Dziedzic, Jennifer L. ; Bucek, Adriana ; Cooper, Leslie T Jr. ; Brandt, Jessica E. ; Alikhan, Fatima S. ; Wang, Haofan ; Endres, Christopher J. ; Choi, Judy ; Pomper, Martin G. ; Guilarte, Tomás R. / Sex differences in translocator protein 18 kDa (TSPO) in the heart : Implications for imaging myocardial inflammation. In: Journal of Cardiovascular Translational Research. 2014 ; Vol. 7, No. 2. pp. 192-202.
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