Sex- and depot-dependent differences in adipogenesis in normal-weight humans

Yourka D. Tchoukalova, Christina Koutsari, Susanne B. Votruba, Tamara Tchkonia, Nino Giorgadze, Thomas Thomou, James L Kirkland, Michael Dennis Jensen

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85 Scopus citations

Abstract

To elucidate cellular mechanisms of sex-related differences in fat distribution, we determined body fat distribution (dual-energy X-ray absorptiometry and single-slice abdominal computed tomography (CT)), adipocyte size, adipocyte number, and proportion of early-differentiated adipocytes (aP2 CD68 ) in the stromovascular fraction (SVF) in the upper and lower body of normal-weight healthy men (n = 12) and premenopausal women (n = 20) (age: 18-49 years, BMI: 18-26kg/m2). Women had more subcutaneous and less visceral fat than men. The proportion of early differentiated adipocytes in the subcutaneous adipose tissue SVF of women was greater than in men (P = 0.01), especially in the femoral depot, although in vitro adipogenesis, as assessed by peroxisome proliferator activated receptor-γ (PPARγ) expression, was not increased in femoral preadipocytes cultured from women compared with men. In women, differentiation of femoral preadipocytes was less than that of abdominal subcutaneous preadipocytes (P = 0.04), and femoral subcutaneous preadipocytes tended to be more resistant to tumor necrosis factor-α (TNFα)-induced apoptosis (P = 0.06). Thus, turnover and utilization of the preadipocyte pool may be reduced in lower vs. the upper-body fat in women. Collectively, these data indicate that the microenvironment, rather than differences in inherent properties of preadipocytes between genders, may explain the gynoid obesity phenotype and higher percent body fat in women compared to men.

Original languageEnglish (US)
Pages (from-to)1875-1880
Number of pages6
JournalObesity
Volume18
Issue number10
DOIs
StatePublished - Oct 2010

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ASJC Scopus subject areas

  • Endocrinology
  • Medicine (miscellaneous)
  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics

Cite this

Tchoukalova, Y. D., Koutsari, C., Votruba, S. B., Tchkonia, T., Giorgadze, N., Thomou, T., Kirkland, J. L., & Jensen, M. D. (2010). Sex- and depot-dependent differences in adipogenesis in normal-weight humans. Obesity, 18(10), 1875-1880. https://doi.org/10.1038/oby.2010.56