Serum of obstructive sleep apnea patients impairs human coronary endothelial cell migration

Michal Hoffmann, Jacek Wolf, Anna Szyndler, Prachi Singh, Virend Somers, Krzysztof Narkiewicz

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Introduction: Endothelial cell migration and proliferation play an important role in the growth and development of new blood vessels and endothelium healing. This process occurs in response to injury, inflammation and immune reactions. Dysfunction of the endothelium may play a significant role in development and progression of cardiovascular disease related to sleep-disordered breathing. The aim of our study was to evaluate the chemo-attractant activity of serum from obstructive sleep apnea (OSA) and normal subjects on coronary artery endothelial cell migration. Material and methods: We studied 12 severe OSA patients, free of other co-morbidities and on no treatment, along with 12 age-, body mass index, and gender matched healthy controls. Blood was collected at three time points: at 21:00 before sleep, at 6:00 after waking from sleep, and at 11:00 (after 5 h of normal daytime activity). Serum chemo-attractant activity for human coronary endothelial cells was assessed using a colorimetric cell migration assay kit. Results: In healthy subjects, serum chemo-attractant activity peaked in the morning after waking from sleep (p = 0.02). This early morning increase was blunted in severe OSA subjects, in whom chemo-attractant activity was weaker than in normal controls (p = 0.02), and did not change significantly at the different time-points (p < 0.001 vs. controls). Conclusions: Chemo-attractant activity of the serum from OSA patients is lower compared to serum from healthy subjects, especially in the morning. Altered chemo-attractant serum activity may conceivably contribute to the impairment of endothelial function in obstructive sleep apnea patients.

Original languageEnglish (US)
Pages (from-to)223-227
Number of pages5
JournalArchives of Medical Science
Volume13
Issue number1
DOIs
StatePublished - Feb 1 2017

Fingerprint

Obstructive Sleep Apnea
Cell Movement
Endothelial Cells
Serum
Sleep
Endothelium
Healthy Volunteers
Cell Migration Assays
Sleep Apnea Syndromes
Growth and Development
Human Activities
Blood Vessels
Coronary Vessels
Body Mass Index
Cardiovascular Diseases
Cell Proliferation
Inflammation
Morbidity
Wounds and Injuries

Keywords

  • Coronary endothelial cell migration
  • Hypoxia
  • Inflammation
  • Obstructive sleep apnea
  • Serum chemo-attractant activity

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Serum of obstructive sleep apnea patients impairs human coronary endothelial cell migration. / Hoffmann, Michal; Wolf, Jacek; Szyndler, Anna; Singh, Prachi; Somers, Virend; Narkiewicz, Krzysztof.

In: Archives of Medical Science, Vol. 13, No. 1, 01.02.2017, p. 223-227.

Research output: Contribution to journalArticle

Hoffmann, Michal ; Wolf, Jacek ; Szyndler, Anna ; Singh, Prachi ; Somers, Virend ; Narkiewicz, Krzysztof. / Serum of obstructive sleep apnea patients impairs human coronary endothelial cell migration. In: Archives of Medical Science. 2017 ; Vol. 13, No. 1. pp. 223-227.
@article{9210e6e5a2cc4c48923a4a4dc7ebe483,
title = "Serum of obstructive sleep apnea patients impairs human coronary endothelial cell migration",
abstract = "Introduction: Endothelial cell migration and proliferation play an important role in the growth and development of new blood vessels and endothelium healing. This process occurs in response to injury, inflammation and immune reactions. Dysfunction of the endothelium may play a significant role in development and progression of cardiovascular disease related to sleep-disordered breathing. The aim of our study was to evaluate the chemo-attractant activity of serum from obstructive sleep apnea (OSA) and normal subjects on coronary artery endothelial cell migration. Material and methods: We studied 12 severe OSA patients, free of other co-morbidities and on no treatment, along with 12 age-, body mass index, and gender matched healthy controls. Blood was collected at three time points: at 21:00 before sleep, at 6:00 after waking from sleep, and at 11:00 (after 5 h of normal daytime activity). Serum chemo-attractant activity for human coronary endothelial cells was assessed using a colorimetric cell migration assay kit. Results: In healthy subjects, serum chemo-attractant activity peaked in the morning after waking from sleep (p = 0.02). This early morning increase was blunted in severe OSA subjects, in whom chemo-attractant activity was weaker than in normal controls (p = 0.02), and did not change significantly at the different time-points (p < 0.001 vs. controls). Conclusions: Chemo-attractant activity of the serum from OSA patients is lower compared to serum from healthy subjects, especially in the morning. Altered chemo-attractant serum activity may conceivably contribute to the impairment of endothelial function in obstructive sleep apnea patients.",
keywords = "Coronary endothelial cell migration, Hypoxia, Inflammation, Obstructive sleep apnea, Serum chemo-attractant activity",
author = "Michal Hoffmann and Jacek Wolf and Anna Szyndler and Prachi Singh and Virend Somers and Krzysztof Narkiewicz",
year = "2017",
month = "2",
day = "1",
doi = "10.5114/aoms.2015.56490",
language = "English (US)",
volume = "13",
pages = "223--227",
journal = "Archives of Medical Science",
issn = "1734-1922",
publisher = "Termedia Publishing House Ltd.",
number = "1",

}

TY - JOUR

T1 - Serum of obstructive sleep apnea patients impairs human coronary endothelial cell migration

AU - Hoffmann, Michal

AU - Wolf, Jacek

AU - Szyndler, Anna

AU - Singh, Prachi

AU - Somers, Virend

AU - Narkiewicz, Krzysztof

PY - 2017/2/1

Y1 - 2017/2/1

N2 - Introduction: Endothelial cell migration and proliferation play an important role in the growth and development of new blood vessels and endothelium healing. This process occurs in response to injury, inflammation and immune reactions. Dysfunction of the endothelium may play a significant role in development and progression of cardiovascular disease related to sleep-disordered breathing. The aim of our study was to evaluate the chemo-attractant activity of serum from obstructive sleep apnea (OSA) and normal subjects on coronary artery endothelial cell migration. Material and methods: We studied 12 severe OSA patients, free of other co-morbidities and on no treatment, along with 12 age-, body mass index, and gender matched healthy controls. Blood was collected at three time points: at 21:00 before sleep, at 6:00 after waking from sleep, and at 11:00 (after 5 h of normal daytime activity). Serum chemo-attractant activity for human coronary endothelial cells was assessed using a colorimetric cell migration assay kit. Results: In healthy subjects, serum chemo-attractant activity peaked in the morning after waking from sleep (p = 0.02). This early morning increase was blunted in severe OSA subjects, in whom chemo-attractant activity was weaker than in normal controls (p = 0.02), and did not change significantly at the different time-points (p < 0.001 vs. controls). Conclusions: Chemo-attractant activity of the serum from OSA patients is lower compared to serum from healthy subjects, especially in the morning. Altered chemo-attractant serum activity may conceivably contribute to the impairment of endothelial function in obstructive sleep apnea patients.

AB - Introduction: Endothelial cell migration and proliferation play an important role in the growth and development of new blood vessels and endothelium healing. This process occurs in response to injury, inflammation and immune reactions. Dysfunction of the endothelium may play a significant role in development and progression of cardiovascular disease related to sleep-disordered breathing. The aim of our study was to evaluate the chemo-attractant activity of serum from obstructive sleep apnea (OSA) and normal subjects on coronary artery endothelial cell migration. Material and methods: We studied 12 severe OSA patients, free of other co-morbidities and on no treatment, along with 12 age-, body mass index, and gender matched healthy controls. Blood was collected at three time points: at 21:00 before sleep, at 6:00 after waking from sleep, and at 11:00 (after 5 h of normal daytime activity). Serum chemo-attractant activity for human coronary endothelial cells was assessed using a colorimetric cell migration assay kit. Results: In healthy subjects, serum chemo-attractant activity peaked in the morning after waking from sleep (p = 0.02). This early morning increase was blunted in severe OSA subjects, in whom chemo-attractant activity was weaker than in normal controls (p = 0.02), and did not change significantly at the different time-points (p < 0.001 vs. controls). Conclusions: Chemo-attractant activity of the serum from OSA patients is lower compared to serum from healthy subjects, especially in the morning. Altered chemo-attractant serum activity may conceivably contribute to the impairment of endothelial function in obstructive sleep apnea patients.

KW - Coronary endothelial cell migration

KW - Hypoxia

KW - Inflammation

KW - Obstructive sleep apnea

KW - Serum chemo-attractant activity

UR - http://www.scopus.com/inward/record.url?scp=85007444089&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85007444089&partnerID=8YFLogxK

U2 - 10.5114/aoms.2015.56490

DO - 10.5114/aoms.2015.56490

M3 - Article

AN - SCOPUS:85007444089

VL - 13

SP - 223

EP - 227

JO - Archives of Medical Science

JF - Archives of Medical Science

SN - 1734-1922

IS - 1

ER -