Serum Cortisol Levels via Radioimmunoassay vs Liquid Chromatography Mass Spectrophotometry in Healthy Control Subjects and Patients With Adrenal Incidentalomas

Martha K.P. Huayllas, Brian C. Netzel, Ravinder Jit Singh, Claudio E. Kater

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: Adrenal incidentalomas (AIs) are present in 4% of adults. As many as 30% may secrete cortisol autonomously in the absence of specific signs of overt hypercortisolism, in a phenomenon called subclinical hypercortisolism (SH). Diagnosis of SH is established by serum cortisol resistance to dexamethasone suppression.

Methods: We compared serum cortisol concentrations, as determined by radioimmunoassay (RIA) and liquid chromatography/tandem mass spectronomy (LC/MS-MS), in 73 patients with AI group (52 with unilateral AI) and 34 control subjects in 3 scenarios: basal; after 1-mg dexamethasone suppression; and after 0.25-mg stimulation with cosyntropin, a synthetic derivative of adrenocorticotropic hormone (ACTH). To bolster evidence for the diagnosis of SH, we also measured salivary cortisol levels at 11 PM and after DST, as well as plasma ACTH and serum dehydroepiandrosterone sulfate (DHEA-S) levels.

Results: We observed significant positive correlation (r = 0.9345, P <.001) for all 318 pairs of serum cortisol values, as measured by both methods.

Conclusions: Serum cortisol concentrations in patients with AI and in control subjects were very similar, as measured by RIA and LC/MS-MS.

Original languageEnglish (US)
Pages (from-to)259-267
Number of pages9
JournalLaboratory Medicine
Volume49
Issue number3
DOIs
StatePublished - Jul 5 2018

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Spectrophotometry
Liquid chromatography
Liquid Chromatography
Radioimmunoassay
Hydrocortisone
Healthy Volunteers
Cushing Syndrome
Serum
Adrenocorticotropic Hormone
Dexamethasone
Cosyntropin
Dehydroepiandrosterone Sulfate
Adrenal incidentaloma
Derivatives
Plasmas

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Serum Cortisol Levels via Radioimmunoassay vs Liquid Chromatography Mass Spectrophotometry in Healthy Control Subjects and Patients With Adrenal Incidentalomas. / Huayllas, Martha K.P.; Netzel, Brian C.; Singh, Ravinder Jit; Kater, Claudio E.

In: Laboratory Medicine, Vol. 49, No. 3, 05.07.2018, p. 259-267.

Research output: Contribution to journalArticle

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