Serotonergic innervation of phrenic motoneurons is increased by cervical dorsal rhizotomy (CDR)

Y.s. Prakash, R. Kinkead, W. Z. Zhan, K. Smithson, P. Zhan, Gary C Sieck, G. S. Mitchell

Research output: Contribution to journalArticle

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Abstract

We hypothesized that bilateral CDR (C3-C5) increases serotonergic innervation of phrenic motoneurons. Surgery on two rat groups (CDR and sham-operated; both N=6) was performed 28 days before sacrifice. Phrenic motoneurons labeled with cholera toxin B-fragment were reconstructed in three dimensions with a Neurolucida Imaging System. Serotonin immunoreactive terminals in immediate proximity to labeled phrenic motoneurons were counted, and terminal densities were calculated using estimates of soma (prolated sphere model) and dendrite (cylindrical model) surface areas based on measurements cell diameter. Absolute #'s Density (#/μ2) CDR Sham-operated CDR Sham-operated Soma 177 ± 10*132 ± 9 0.11 ± 0.01 0.12 ± 0.01 Dendrites 103 ± 20*70 ± 11 0.52 ± 0.08 0.49 ± 0.01*: vs. Sham-operated rats (p < 0.05). We conclude that CDR increases serotonergic innervation of the phrenic motor nucleus, maintaining terminal density despite enlarged motoneuron size (Zhan et al., ibid).

Original languageEnglish (US)
JournalFASEB Journal
Volume11
Issue number3
StatePublished - 1997
Externally publishedYes

Fingerprint

Rhizotomy
Carisoprodol
Motor Neurons
innervation
motor neurons
Diaphragm
Rats
Cholera Toxin
dendrites
Imaging systems
Surgery
Serotonin
Dendrites
cholera toxin
rats
serotonin
surface area
surgery
image analysis
cells

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Prakash, Y. S., Kinkead, R., Zhan, W. Z., Smithson, K., Zhan, P., Sieck, G. C., & Mitchell, G. S. (1997). Serotonergic innervation of phrenic motoneurons is increased by cervical dorsal rhizotomy (CDR). FASEB Journal, 11(3).

Serotonergic innervation of phrenic motoneurons is increased by cervical dorsal rhizotomy (CDR). / Prakash, Y.s.; Kinkead, R.; Zhan, W. Z.; Smithson, K.; Zhan, P.; Sieck, Gary C; Mitchell, G. S.

In: FASEB Journal, Vol. 11, No. 3, 1997.

Research output: Contribution to journalArticle

Prakash, YS, Kinkead, R, Zhan, WZ, Smithson, K, Zhan, P, Sieck, GC & Mitchell, GS 1997, 'Serotonergic innervation of phrenic motoneurons is increased by cervical dorsal rhizotomy (CDR)', FASEB Journal, vol. 11, no. 3.
Prakash, Y.s. ; Kinkead, R. ; Zhan, W. Z. ; Smithson, K. ; Zhan, P. ; Sieck, Gary C ; Mitchell, G. S. / Serotonergic innervation of phrenic motoneurons is increased by cervical dorsal rhizotomy (CDR). In: FASEB Journal. 1997 ; Vol. 11, No. 3.
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AU - Mitchell, G. S.

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