Ia specificities 22 and 23 were found to be determinants on hybrid Ia molecules by serological and biochemical studies. Lipopolysaccharide-stimulated splenic lymphocytes from (B10 x B10.D2)F1 expressed Ia.22 although both the parents were negative. Similarly [D2.GD x B10.A(5R)]F1 cells expressed Ia.23, whereas D2.GD and B10.A(5R) lacked it. Ia.22 can be generated by gene complementation of A(k)-E(k), A(b)-E(d), A(b)-E(k), A(s)-E(d), and A(s)-E(k), whereas Ia.23 can be generated by A(d)-E(d), A(d)-E(k), and A(d)-E(p). Other possible complementing combinations are under study. The role of Ia.22 and 23 in mixed lymphocyte reactions and immune responses are discussed.
ASJC Scopus subject areas
- Immunology and Allergy