Sequential G-banding and fluorescent in situ hybridization on peripheral blood, bone marrow, and amniotic fluid samples

Anne Wiktor; Regina Puskorius; Kristina Zubrickas; Daniel L. Van Dyke

doi:10.1002/(SICI)1096-8628(19980827)79:1<38::AID-AJMG10>3.0.CO;2-N

Sequential G-banding and fluorescent in situ hybridization on peripheral blood, bone marrow, and amniotic fluid samples

Anne Wiktor, Regina Puskorius, Kristina Zubrickas, Daniel L. Van Dyke

Laboratory Medicine and Pathology

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

We describe a method for fluorescent in situ hybridization (FISH) on previously G-banded slides and mounted coverslip preparations. The technique is successful on previously G-banded slides from peripheral blood and bone marrow specimens and on amniotic fluid samples from in situ harvests. Satellite sequence probes, whole chromosome paints, and unique sequence probes have all produced reliable results.

Original language	English (US)
Pages (from-to)	38-41
Number of pages	4
Journal	American journal of medical genetics
Volume	79
Issue number	1
DOIs	https://doi.org/10.1002/(SICI)1096-8628(19980827)79:1<38::AID-AJMG10>3.0.CO;2-N
State	Published - 1998

Keywords

FISH
Fluorescent in situ hybridization
G-banding
Sequential banding

ASJC Scopus subject areas

Genetics
Genetics(clinical)

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10.1002/(SICI)1096-8628(19980827)79:1<38::AID-AJMG10>3.0.CO;2-N

Cite this

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AU - Wiktor, Anne

AU - Puskorius, Regina

AU - Zubrickas, Kristina

AU - Van Dyke, Daniel L.

PY - 1998

Y1 - 1998

N2 - We describe a method for fluorescent in situ hybridization (FISH) on previously G-banded slides and mounted coverslip preparations. The technique is successful on previously G-banded slides from peripheral blood and bone marrow specimens and on amniotic fluid samples from in situ harvests. Satellite sequence probes, whole chromosome paints, and unique sequence probes have all produced reliable results.

AB - We describe a method for fluorescent in situ hybridization (FISH) on previously G-banded slides and mounted coverslip preparations. The technique is successful on previously G-banded slides from peripheral blood and bone marrow specimens and on amniotic fluid samples from in situ harvests. Satellite sequence probes, whole chromosome paints, and unique sequence probes have all produced reliable results.

KW - FISH

KW - Fluorescent in situ hybridization

KW - G-banding

KW - Sequential banding

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ER -

Sequential G-banding and fluorescent in situ hybridization on peripheral blood, bone marrow, and amniotic fluid samples

Abstract

Keywords

ASJC Scopus subject areas

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