Sequential delivery of dexamethasone and VEGF to control local tissue response for carbon nanotube fluorescence based micro-capillary implantable sensors

Jaeyun Sung, Paul W. Barone, Hyunjoon Kong, Michael S. Strano

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

In this study, we examined the in vivo pharmacological effects of the sequential delivery of dexamethasone (DX) followed by vascular endothelial growth factor (VEGF) on the immune response and localized vascular network formation around a hydrogel-coated, micro-capillary implant for single-walled carbon nanotube based fluorescence sensors. We demonstrate, for the first time, imaging of an SWNT fluorescence device implanted subcutaneously in a rat. For tissue response studies, the chick embryo chorioallantoic membrane (CAM) was used as a tissue-model for an 8-day implantation period. The average vascular density of the tissue surrounding a hydrogel-coated microdialysis capillary sensor with simultaneous, sequential, or no delivery of DX and VEGF was 1.24 ± 0.35 × 10-3 vessels/μm2, 1.15 ± 0.30 × 10-3 vessels/μm2 and 0.71 ± 0.20 × 10-3 vessels/μm2, respectively. Calculation of the therapeutic index (vasculature/inflammation ratio), which reflects promotion of angiogenesis versus the host immune response, demonstrates that sequential DX/VEGF delivery was 60.3% and 139.3% higher than that of VEGF and DX release alone, respectively, and was also 32.1% higher when compared to simultaneous administration, proving to be a more effective strategy in utilizing the pharmacological impact of DX and VEGF around the biosensor-model implant.

Original languageEnglish (US)
Pages (from-to)622-631
Number of pages10
JournalBiomaterials
Volume30
Issue number4
DOIs
StatePublished - Feb 2009
Externally publishedYes

Fingerprint

Carbon Nanotubes
Dexamethasone
Vascular Endothelial Growth Factor A
Carbon nanotubes
Fluorescence
Tissue
Sensors
Hydrogel
Hydrogels
Blood Vessels
Pharmacology
Chorioallantoic Membrane
Optical Imaging
Microdialysis
Biosensing Techniques
Single-walled carbon nanotubes (SWCN)
Chick Embryo
Biosensors
Rats
Intercellular Signaling Peptides and Proteins

Keywords

  • Angiogenesis
  • Biosensor
  • Drug delivery
  • Immune response

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Cite this

Sequential delivery of dexamethasone and VEGF to control local tissue response for carbon nanotube fluorescence based micro-capillary implantable sensors. / Sung, Jaeyun; Barone, Paul W.; Kong, Hyunjoon; Strano, Michael S.

In: Biomaterials, Vol. 30, No. 4, 02.2009, p. 622-631.

Research output: Contribution to journalArticle

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