Sequencing and bacterial expression of a novel murine alpha interferon gene

Yan Wang, Zhengyu Wang, Mingnan Zhou, Ju'e Cai, Lanying Sun, Xinyuan Liu, B. L. Daugherty, S. Pestka

Research output: Contribution to journalArticle

Abstract

A murine new alpha interferon gene (mIFN-αB) was found by primer-based sequencing method in a murine genomic DNA library. The gene was cloned and its sequence was determined. It was expressed in Escherichia coli under the control of the PL promoter which resulted in antiviral activity on mouse L-cells. The sequence of mIFN-αB has been accepted by GENEBANK.

Original languageEnglish (US)
Pages (from-to)277-283
Number of pages7
JournalScience in China, Series C: Life Sciences
Volume40
Issue number3
DOIs
StatePublished - Jan 1 1997

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Keywords

  • Murine alpha interferon
  • Primer-based sequencing method
  • Prokaryotic expression

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Agricultural and Biological Sciences(all)

Cite this

Wang, Y., Wang, Z., Zhou, M., Cai, J., Sun, L., Liu, X., Daugherty, B. L., & Pestka, S. (1997). Sequencing and bacterial expression of a novel murine alpha interferon gene. Science in China, Series C: Life Sciences, 40(3), 277-283. https://doi.org/10.1007/BF02879088