Sequencing and bacterial expression of a novel murine alpha interferon gene

Yan Wang; Zhengyu Wang; Mingnan Zhou; Ju'e Cai; Lanying Sun; Xinyuan Liu; B. L. Daugherty; S. Pestka

doi:10.1007/BF02879088

Sequencing and bacterial expression of a novel murine alpha interferon gene

Yan Wang, Zhengyu Wang, Mingnan Zhou, Ju'e Cai, Lanying Sun, Xinyuan Liu, B. L. Daugherty, S. Pestka

Quantitative Health Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

A murine new alpha interferon gene (mIFN-αB) was found by primer-based sequencing method in a murine genomic DNA library. The gene was cloned and its sequence was determined. It was expressed in Escherichia coli under the control of the P_L promoter which resulted in antiviral activity on mouse L-cells. The sequence of mIFN-αB has been accepted by GENEBANK.

Original language	English (US)
Pages (from-to)	277-283
Number of pages	7
Journal	Science in China, Series C: Life Sciences
Volume	40
Issue number	3
DOIs	https://doi.org/10.1007/BF02879088
State	Published - 1997

Keywords

Murine alpha interferon
Primer-based sequencing method
Prokaryotic expression

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology
General Environmental Science
General Agricultural and Biological Sciences

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10.1007/BF02879088

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abstract = "A murine new alpha interferon gene (mIFN-αB) was found by primer-based sequencing method in a murine genomic DNA library. The gene was cloned and its sequence was determined. It was expressed in Escherichia coli under the control of the PL promoter which resulted in antiviral activity on mouse L-cells. The sequence of mIFN-αB has been accepted by GENEBANK.",

keywords = "Murine alpha interferon, Primer-based sequencing method, Prokaryotic expression",

author = "Yan Wang and Zhengyu Wang and Mingnan Zhou and Ju'e Cai and Lanying Sun and Xinyuan Liu and Daugherty, {B. L.} and S. Pestka",

year = "1997",

doi = "10.1007/BF02879088",

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AU - Wang, Yan

AU - Wang, Zhengyu

AU - Zhou, Mingnan

AU - Cai, Ju'e

AU - Sun, Lanying

AU - Liu, Xinyuan

AU - Daugherty, B. L.

AU - Pestka, S.

PY - 1997

Y1 - 1997

N2 - A murine new alpha interferon gene (mIFN-αB) was found by primer-based sequencing method in a murine genomic DNA library. The gene was cloned and its sequence was determined. It was expressed in Escherichia coli under the control of the PL promoter which resulted in antiviral activity on mouse L-cells. The sequence of mIFN-αB has been accepted by GENEBANK.

AB - A murine new alpha interferon gene (mIFN-αB) was found by primer-based sequencing method in a murine genomic DNA library. The gene was cloned and its sequence was determined. It was expressed in Escherichia coli under the control of the PL promoter which resulted in antiviral activity on mouse L-cells. The sequence of mIFN-αB has been accepted by GENEBANK.

KW - Murine alpha interferon

KW - Primer-based sequencing method

KW - Prokaryotic expression

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JO - Science in China, Series C: Life Sciences

JF - Science in China, Series C: Life Sciences

IS - 3

ER -

Sequencing and bacterial expression of a novel murine alpha interferon gene

Abstract

Keywords

ASJC Scopus subject areas

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