Sequence specificity of cyclobutane pyrimidine dimers in DNA treated with solar (ultraviolet B) radiation

David L. Mitchell; Jin Jen; James E. Cleaver

doi:10.1093/nar/20.2.225

Sequence specificity of cyclobutane pyrimidine dimers in DNA treated with solar (ultraviolet B) radiation

David L. Mitchell, Jin Jen, James E. Cleaver

Laboratory Medicine and Pathology

Research output: Contribution to journal › Article

155 Scopus citations

Abstract

Cyclobutane pyrimidine dimers were quantified at the sequence level after irradiation with solar ultraviolet (UVB) and nonsolar ultraviolet (UVC) light sources. The yield of photoproducts at specific sites was dependent on the nucleotide composition in and around the potential lesion as well as on the wavelength of ultraviolet light used to induce the damage. Induction was greater in the presence of 5' flanking pyromidines than purines; 5' guanine inhibited induction more than adenine. UVB irradiation increased the induction of cyclobutane dimers containing cytosine relative to thymine homodimers. At the single UVC and UVB fluences used, the ratio of thymine homodimers (T< >T) to dimers containing cytosine (C< >T, T< >C, C< >C) was greater after UVC compared to UVB irradiation.

Original language	English (US)
Pages (from-to)	225-229
Number of pages	5
Journal	Nucleic acids research
Volume	20
Issue number	2
DOIs	https://doi.org/10.1093/nar/20.2.225
State	Published - Jan 25 1992

ASJC Scopus subject areas

Genetics

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Mitchell, D. L., Jen, J., & Cleaver, J. E. (1992). Sequence specificity of cyclobutane pyrimidine dimers in DNA treated with solar (ultraviolet B) radiation. Nucleic acids research, 20(2), 225-229. https://doi.org/10.1093/nar/20.2.225

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abstract = "Cyclobutane pyrimidine dimers were quantified at the sequence level after irradiation with solar ultraviolet (UVB) and nonsolar ultraviolet (UVC) light sources. The yield of photoproducts at specific sites was dependent on the nucleotide composition in and around the potential lesion as well as on the wavelength of ultraviolet light used to induce the damage. Induction was greater in the presence of 5' flanking pyromidines than purines; 5' guanine inhibited induction more than adenine. UVB irradiation increased the induction of cyclobutane dimers containing cytosine relative to thymine homodimers. At the single UVC and UVB fluences used, the ratio of thymine homodimers (T< >T) to dimers containing cytosine (C< >T, T< >C, C< >C) was greater after UVC compared to UVB irradiation.",

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