Abstract
Cyclobutane pyrimidine dimers were quantified at the sequence level after irradiation with solar ultraviolet (UVB) and nonsolar ultraviolet (UVC) light sources. The yield of photoproducts at specific sites was dependent on the nucleotide composition in and around the potential lesion as well as on the wavelength of ultraviolet light used to induce the damage. Induction was greater in the presence of 5′ flanking pyrimidines then purines; 5′ guanine inhibited induction more than adenine. UVB irradiation increased the induction of cyclobutane dimers containing cytosine relative to thymine homodimers. At the single UVC and UVB fluences used, the ratio of thymine homodimers (T< >T) to dimers containing cytosine (C< >T, T< >C, C< >C) was greater after UVC compared to UVB irradiation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 225-229 |
| Number of pages | 5 |
| Journal | Nucleic Acids Research |
| Volume | 20 |
| Issue number | 2 |
| State | Published - 1992 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Genetics
- Statistics, Probability and Uncertainty
- Applied Mathematics
- Health, Toxicology and Mutagenesis
- Toxicology
- Genetics(clinical)
Cite this
Sequence specificity of cyclobutane pyrimidine dimers in DNA treated with solar (ultraviolet B) radiation. / Mitchell, David L.; Jen, Jin; Cleaver, James E.
In: Nucleic Acids Research, Vol. 20, No. 2, 1992, p. 225-229.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Sequence specificity of cyclobutane pyrimidine dimers in DNA treated with solar (ultraviolet B) radiation
AU - Mitchell, David L.
AU - Jen, Jin
AU - Cleaver, James E.
PY - 1992
Y1 - 1992
N2 - Cyclobutane pyrimidine dimers were quantified at the sequence level after irradiation with solar ultraviolet (UVB) and nonsolar ultraviolet (UVC) light sources. The yield of photoproducts at specific sites was dependent on the nucleotide composition in and around the potential lesion as well as on the wavelength of ultraviolet light used to induce the damage. Induction was greater in the presence of 5′ flanking pyrimidines then purines; 5′ guanine inhibited induction more than adenine. UVB irradiation increased the induction of cyclobutane dimers containing cytosine relative to thymine homodimers. At the single UVC and UVB fluences used, the ratio of thymine homodimers (T< >T) to dimers containing cytosine (C< >T, T< >C, C< >C) was greater after UVC compared to UVB irradiation.
AB - Cyclobutane pyrimidine dimers were quantified at the sequence level after irradiation with solar ultraviolet (UVB) and nonsolar ultraviolet (UVC) light sources. The yield of photoproducts at specific sites was dependent on the nucleotide composition in and around the potential lesion as well as on the wavelength of ultraviolet light used to induce the damage. Induction was greater in the presence of 5′ flanking pyrimidines then purines; 5′ guanine inhibited induction more than adenine. UVB irradiation increased the induction of cyclobutane dimers containing cytosine relative to thymine homodimers. At the single UVC and UVB fluences used, the ratio of thymine homodimers (T< >T) to dimers containing cytosine (C< >T, T< >C, C< >C) was greater after UVC compared to UVB irradiation.
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UR - http://www.scopus.com/inward/citedby.url?scp=0026551941&partnerID=8YFLogxK
M3 - Article
VL - 20
SP - 225
EP - 229
JO - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
IS - 2
ER -