Sequence of human eosinophil-derived neurotoxin cDNA: identity of deduced amino acid sequence with human nonsecretory ribonucleases

Kimm J. Hamann; Robert L. Barker; David A. Loegering; Larry R. Pease; Gerald J. Gleich

doi:10.1016/0378-1119(89)90414-9

Sequence of human eosinophil-derived neurotoxin cDNA: identity of deduced amino acid sequence with human nonsecretory ribonucleases

Kimm J. Hamann, Robert L. Barker, David A. Loegering, Larry R. Pease, Gerald J. Gleich

Immunology

Research output: Contribution to journal › Article › peer-review

62 Scopus citations

Abstract

Several clones of human eosinophil-derived neurotoxin (EDN) cDNA have been isolated from a λgt10 cDNA library prepared from mRNA derived from noninduced HL-60 cells. The amino acid (aa) sequence deduced from the coding sequence of the EDN cDNA is identical to the aa sequence of urinary nonsecretory RNase. Comparison of the aa and/or nucleotide (nt) sequences of EDN and other proteins possessing ribonucleolytic activity, namely bovine seminal RNase, human and rat pancreatic RNases, eosinophil cationic protein (ECP), and human angiogenin, shows extensive identity at half-cystine residues and at aa of active sites. Differences in aa sequences at the active sites are often the result of single nt changes in the codons. The data presented here support the concept of a RNase gene superfamily containing secretory and nonsecretory RNases, angiogenin, EDN and ECP.

Original language	English (US)
Pages (from-to)	161-167
Number of pages	7
Journal	Gene
Volume	83
Issue number	1
DOIs	https://doi.org/10.1016/0378-1119(89)90414-9
State	Published - Nov 15 1989

Keywords

Angiogenin
cationic protein
gene superfamily
human urinary RNase
nucleotide sequence
phage λ library

ASJC Scopus subject areas

Genetics

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10.1016/0378-1119(89)90414-9

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title = "Sequence of human eosinophil-derived neurotoxin cDNA: identity of deduced amino acid sequence with human nonsecretory ribonucleases",

abstract = "Several clones of human eosinophil-derived neurotoxin (EDN) cDNA have been isolated from a λgt10 cDNA library prepared from mRNA derived from noninduced HL-60 cells. The amino acid (aa) sequence deduced from the coding sequence of the EDN cDNA is identical to the aa sequence of urinary nonsecretory RNase. Comparison of the aa and/or nucleotide (nt) sequences of EDN and other proteins possessing ribonucleolytic activity, namely bovine seminal RNase, human and rat pancreatic RNases, eosinophil cationic protein (ECP), and human angiogenin, shows extensive identity at half-cystine residues and at aa of active sites. Differences in aa sequences at the active sites are often the result of single nt changes in the codons. The data presented here support the concept of a RNase gene superfamily containing secretory and nonsecretory RNases, angiogenin, EDN and ECP.",

keywords = "Angiogenin, cationic protein, gene superfamily, human urinary RNase, nucleotide sequence, phage λ library",

author = "Hamann, {Kimm J.} and Barker, {Robert L.} and Loegering, {David A.} and Pease, {Larry R.} and Gleich, {Gerald J.}",

note = "Funding Information: We thank Drs. Rosa Ten and David McKean for technical advice and helpful discussions and Linda H. Arneson for her secretariala ssistance.T his work was supported in part by grants from the National Institutes of Health, AI 09728 and AI 07047, and by the Mayo Foundation. The nt sequencer eported in this paper has been submitted to the GenBank/ EMBL Data Bank, with the accession number x13015.",

year = "1989",

month = nov,

day = "15",

doi = "10.1016/0378-1119(89)90414-9",

language = "English (US)",

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pages = "161--167",

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T1 - Sequence of human eosinophil-derived neurotoxin cDNA

T2 - identity of deduced amino acid sequence with human nonsecretory ribonucleases

AU - Hamann, Kimm J.

AU - Barker, Robert L.

AU - Loegering, David A.

AU - Pease, Larry R.

AU - Gleich, Gerald J.

N1 - Funding Information: We thank Drs. Rosa Ten and David McKean for technical advice and helpful discussions and Linda H. Arneson for her secretariala ssistance.T his work was supported in part by grants from the National Institutes of Health, AI 09728 and AI 07047, and by the Mayo Foundation. The nt sequencer eported in this paper has been submitted to the GenBank/ EMBL Data Bank, with the accession number x13015.

PY - 1989/11/15

Y1 - 1989/11/15

N2 - Several clones of human eosinophil-derived neurotoxin (EDN) cDNA have been isolated from a λgt10 cDNA library prepared from mRNA derived from noninduced HL-60 cells. The amino acid (aa) sequence deduced from the coding sequence of the EDN cDNA is identical to the aa sequence of urinary nonsecretory RNase. Comparison of the aa and/or nucleotide (nt) sequences of EDN and other proteins possessing ribonucleolytic activity, namely bovine seminal RNase, human and rat pancreatic RNases, eosinophil cationic protein (ECP), and human angiogenin, shows extensive identity at half-cystine residues and at aa of active sites. Differences in aa sequences at the active sites are often the result of single nt changes in the codons. The data presented here support the concept of a RNase gene superfamily containing secretory and nonsecretory RNases, angiogenin, EDN and ECP.

AB - Several clones of human eosinophil-derived neurotoxin (EDN) cDNA have been isolated from a λgt10 cDNA library prepared from mRNA derived from noninduced HL-60 cells. The amino acid (aa) sequence deduced from the coding sequence of the EDN cDNA is identical to the aa sequence of urinary nonsecretory RNase. Comparison of the aa and/or nucleotide (nt) sequences of EDN and other proteins possessing ribonucleolytic activity, namely bovine seminal RNase, human and rat pancreatic RNases, eosinophil cationic protein (ECP), and human angiogenin, shows extensive identity at half-cystine residues and at aa of active sites. Differences in aa sequences at the active sites are often the result of single nt changes in the codons. The data presented here support the concept of a RNase gene superfamily containing secretory and nonsecretory RNases, angiogenin, EDN and ECP.

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KW - cationic protein

KW - gene superfamily

KW - human urinary RNase

KW - nucleotide sequence

KW - phage λ library

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Sequence of human eosinophil-derived neurotoxin cDNA: identity of deduced amino acid sequence with human nonsecretory ribonucleases

Abstract

Keywords

ASJC Scopus subject areas

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