Separation and suppression of coherent transfer effects in two-dimensional NOE and chemical exchange spectroscopy

Slobodan I Macura, K. Wuthrich, R. R. Ernst

Research output: Contribution to journalArticle

199 Citations (Scopus)

Abstract

A new method for the separation of coherent and incoherent magnetization transfer in two-dimensional (2D) NOE and chemical exchange spectroscopy is described. The new experiment differs from previous 2D exchange experiments, in that the mixing time τm is incremented systematically together with the evolution time t1. This permits the distinction of the different orders of multiple-quantum coherence during the mixing time and allows the separation of coherent and incoherent transfer processes. The resulting clarification of chemical exchange and 2D NOE spectra is illustrated with experiments on small molecules and on a globular protein, the basic pancreatic trypsin inhibitor.

Original languageEnglish (US)
Pages (from-to)269-282
Number of pages14
JournalJournal of Magnetic Resonance (1969)
Volume46
Issue number2
DOIs
StatePublished - Feb 1 1982
Externally publishedYes

Fingerprint

Ion exchange
Spectroscopy
Aprotinin
Experiments
Magnetization
Molecules
Proteins

Cite this

Separation and suppression of coherent transfer effects in two-dimensional NOE and chemical exchange spectroscopy. / Macura, Slobodan I; Wuthrich, K.; Ernst, R. R.

In: Journal of Magnetic Resonance (1969), Vol. 46, No. 2, 01.02.1982, p. 269-282.

Research output: Contribution to journalArticle

@article{e2ef0e8286fb458ca1dd926977536f3d,
title = "Separation and suppression of coherent transfer effects in two-dimensional NOE and chemical exchange spectroscopy",
abstract = "A new method for the separation of coherent and incoherent magnetization transfer in two-dimensional (2D) NOE and chemical exchange spectroscopy is described. The new experiment differs from previous 2D exchange experiments, in that the mixing time τm is incremented systematically together with the evolution time t1. This permits the distinction of the different orders of multiple-quantum coherence during the mixing time and allows the separation of coherent and incoherent transfer processes. The resulting clarification of chemical exchange and 2D NOE spectra is illustrated with experiments on small molecules and on a globular protein, the basic pancreatic trypsin inhibitor.",
author = "Macura, {Slobodan I} and K. Wuthrich and Ernst, {R. R.}",
year = "1982",
month = "2",
day = "1",
doi = "10.1016/0022-2364(82)90142-1",
language = "English (US)",
volume = "46",
pages = "269--282",
journal = "Journal of Magnetic Resonance (1969)",
issn = "0022-2364",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Separation and suppression of coherent transfer effects in two-dimensional NOE and chemical exchange spectroscopy

AU - Macura, Slobodan I

AU - Wuthrich, K.

AU - Ernst, R. R.

PY - 1982/2/1

Y1 - 1982/2/1

N2 - A new method for the separation of coherent and incoherent magnetization transfer in two-dimensional (2D) NOE and chemical exchange spectroscopy is described. The new experiment differs from previous 2D exchange experiments, in that the mixing time τm is incremented systematically together with the evolution time t1. This permits the distinction of the different orders of multiple-quantum coherence during the mixing time and allows the separation of coherent and incoherent transfer processes. The resulting clarification of chemical exchange and 2D NOE spectra is illustrated with experiments on small molecules and on a globular protein, the basic pancreatic trypsin inhibitor.

AB - A new method for the separation of coherent and incoherent magnetization transfer in two-dimensional (2D) NOE and chemical exchange spectroscopy is described. The new experiment differs from previous 2D exchange experiments, in that the mixing time τm is incremented systematically together with the evolution time t1. This permits the distinction of the different orders of multiple-quantum coherence during the mixing time and allows the separation of coherent and incoherent transfer processes. The resulting clarification of chemical exchange and 2D NOE spectra is illustrated with experiments on small molecules and on a globular protein, the basic pancreatic trypsin inhibitor.

UR - http://www.scopus.com/inward/record.url?scp=0001591905&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0001591905&partnerID=8YFLogxK

U2 - 10.1016/0022-2364(82)90142-1

DO - 10.1016/0022-2364(82)90142-1

M3 - Article

AN - SCOPUS:0001591905

VL - 46

SP - 269

EP - 282

JO - Journal of Magnetic Resonance (1969)

JF - Journal of Magnetic Resonance (1969)

SN - 0022-2364

IS - 2

ER -