Separation and suppression of coherent transfer effects in two-dimensional NOE and chemical exchange spectroscopy

S. Macura, K. Wuthrich, R. R. Ernst

Research output: Contribution to journalArticle

200 Scopus citations

Abstract

A new method for the separation of coherent and incoherent magnetization transfer in two-dimensional (2D) NOE and chemical exchange spectroscopy is described. The new experiment differs from previous 2D exchange experiments, in that the mixing time τm is incremented systematically together with the evolution time t1. This permits the distinction of the different orders of multiple-quantum coherence during the mixing time and allows the separation of coherent and incoherent transfer processes. The resulting clarification of chemical exchange and 2D NOE spectra is illustrated with experiments on small molecules and on a globular protein, the basic pancreatic trypsin inhibitor.

Original languageEnglish (US)
Pages (from-to)269-282
Number of pages14
JournalJournal of Magnetic Resonance (1969)
Volume46
Issue number2
DOIs
StatePublished - Feb 1 1982

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