Sensory neuron targeting by self-complementary AAV8 via lumbar puncture for chronic pain

Benjamin Storek, Matthias Reinhardt, Cheng Wang, William G M Janssen, Nina M. Harder, Michaela S. Banck, John H. Morrison, Andreas S Beutler

Research output: Contribution to journalArticle

100 Citations (Scopus)

Abstract

Lumbar puncture (LP) is an attractive route to deliver drugs to the nervous system because it is a safe bedside procedure. Its use for gene therapy has been complicated by poor vector performance and failure to target neurons. Here we report highly effective gene transfer to the primary sensory neurons of the dorsal root ganglia (DRGs) with self-complementary recombinant adeno-associated virus serotype 8 (sc-rAAV8) modeling an LP. Transgene expression was selective for these neurons outlining their cell bodies in the DRGs and their axons projecting into the spinal cord. Immunohistochemical studies demonstrated transduction of cells positive for the nociceptive neuron marker vanilloid receptor subtype 1, the small peptidergic neuron markers substance P and calcitonin generelated peptide, and the nonpeptidergic neuron marker griffonia simplicifolia isolectin B4. We tested the efficacy of the approach in a rat model of chronic neuropathic pain. A single administration of sc-rAAV8 expressing the analgesic gene prepro-β-endorphin (ppβEP) led to significant (P < 0.0001) reversal of mechanical allodynia for >3 months. The antiallodynic effect could be reversed by the μ-opioid antagonist naloxone 4 months after gene transfer (P < 0.001). Testing of an alternative nonopioid analgesic gene, IL-10, alone or in combination with ppβEP was equally effective (P < 0.0001). All aspects of the procedure, such as the use of an atraumatic injection technique, isotonic diluent, a low-infusion pressure, and a small injection volume, are consistent with clinical practice of intrathecal drug use. Therefore, gene transfer by LP may be suitable for developing gene therapy-based treatments for chronic pain.

Original languageEnglish (US)
Pages (from-to)1055-1060
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume105
Issue number3
DOIs
StatePublished - Jan 22 2008
Externally publishedYes

Fingerprint

Spinal Puncture
Sensory Receptor Cells
Chronic Pain
Endorphins
Neurons
Dependovirus
Genes
Spinal Ganglia
Genetic Therapy
Non-Narcotic Analgesics
Nociceptors
Injections
Narcotic Antagonists
Calcitonin
Neuralgia
Substance P
Naloxone
Transgenes
Pharmaceutical Preparations
Interleukin-10

Keywords

  • β-endorphin
  • Adeno-associated virus
  • Dorsal root ganglion
  • Gene therapy
  • IL-10

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Sensory neuron targeting by self-complementary AAV8 via lumbar puncture for chronic pain. / Storek, Benjamin; Reinhardt, Matthias; Wang, Cheng; Janssen, William G M; Harder, Nina M.; Banck, Michaela S.; Morrison, John H.; Beutler, Andreas S.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, No. 3, 22.01.2008, p. 1055-1060.

Research output: Contribution to journalArticle

Storek, Benjamin ; Reinhardt, Matthias ; Wang, Cheng ; Janssen, William G M ; Harder, Nina M. ; Banck, Michaela S. ; Morrison, John H. ; Beutler, Andreas S. / Sensory neuron targeting by self-complementary AAV8 via lumbar puncture for chronic pain. In: Proceedings of the National Academy of Sciences of the United States of America. 2008 ; Vol. 105, No. 3. pp. 1055-1060.
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