TY - JOUR
T1 - Senescent cells in human adipose tissue
T2 - A cross-sectional study
AU - Espinosa De Ycaza, Ana Elena
AU - Søndergaard, Esben
AU - Morgan-Bathke, Maria
AU - Carranza Leon, Barbara Gisella
AU - Lytle, Kelli A.
AU - Ramos, Paola
AU - Kirkland, James L.
AU - Tchkonia, Tamar
AU - Jensen, Michael D.
N1 - Publisher Copyright:
© 2021 The Obesity Society
PY - 2021/8
Y1 - 2021/8
N2 - Objective: Adipose tissue (AT) senescence is associated with AT dysfunction in rodents, but little is known about human AT senescence. The study goal was to define the distribution of senescent cells in two subcutaneous depots and understand relationships with adiposity and inflammation. Methods: Sixty-three volunteers (48 females) underwent abdominal and femoral subcutaneous fat biopsies. Fat cell size, senescent cells using senescence-associated β-galactosidase staining per 100 nucleated cells (percentage), and mRNA expression of four cytokines were measured. Results: There was a larger proportion of senescent cells in femoral than abdominal subcutaneous AT (mean difference 1.6% [95% CI: 0.98%-2.3%], p < 0.001), and the percentage of femoral AT senescent cells was greater in women than men (median 3.9% vs. 2.1%, p < 0.01). There was a positive correlation between senescence and fat cell size in abdominal (rs = 0.44, p < 0.001) and femoral (rs = 0.35, p = 0.007) AT depots. Abdominal AT tumor necrosis factor alpha (rs = 0.49, p < 0.01) and interleukin-1β (rs = 0.44, p = 0.01) expression was positively correlated with abdominal, but not femoral, AT senescence. Conclusions: In human subcutaneous AT, there are more senescent cells in femoral than abdominal depots; abdominal AT senescent cells are more associated with inflammatory signals than femoral AT senescent cells.
AB - Objective: Adipose tissue (AT) senescence is associated with AT dysfunction in rodents, but little is known about human AT senescence. The study goal was to define the distribution of senescent cells in two subcutaneous depots and understand relationships with adiposity and inflammation. Methods: Sixty-three volunteers (48 females) underwent abdominal and femoral subcutaneous fat biopsies. Fat cell size, senescent cells using senescence-associated β-galactosidase staining per 100 nucleated cells (percentage), and mRNA expression of four cytokines were measured. Results: There was a larger proportion of senescent cells in femoral than abdominal subcutaneous AT (mean difference 1.6% [95% CI: 0.98%-2.3%], p < 0.001), and the percentage of femoral AT senescent cells was greater in women than men (median 3.9% vs. 2.1%, p < 0.01). There was a positive correlation between senescence and fat cell size in abdominal (rs = 0.44, p < 0.001) and femoral (rs = 0.35, p = 0.007) AT depots. Abdominal AT tumor necrosis factor alpha (rs = 0.49, p < 0.01) and interleukin-1β (rs = 0.44, p = 0.01) expression was positively correlated with abdominal, but not femoral, AT senescence. Conclusions: In human subcutaneous AT, there are more senescent cells in femoral than abdominal depots; abdominal AT senescent cells are more associated with inflammatory signals than femoral AT senescent cells.
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U2 - 10.1002/oby.23202
DO - 10.1002/oby.23202
M3 - Article
C2 - 34114359
AN - SCOPUS:85107464528
SN - 1930-7381
VL - 29
SP - 1320
EP - 1327
JO - Obesity
JF - Obesity
IS - 8
ER -