Semiautomated quantification of β-zone parapapillary atrophy using blue light fundus autofluorescence

Kaweh Mansouri, Jonathan D. Tung, Felipe A. Medeiros, Ali Tafreshi, Syril Dorairaj, Linda Zangwill, Feng He, Sonia Jain, Robert N. Weinreb

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Purpose: To evaluate the reproducibility of measurements of area of β-zone parapapillary atrophy (β-PPA) using blue laser fundus autofluorescence (FAF) and confocal scanning laser ophthalmoscopy reflectance (CSLO) measurements and to assess agreement between the two imaging modalities. Methods: Sixty-five eyes of 45 patients (mean age, 68.2 ± 11.3 years) with established or suspected glaucoma from the Diagnostic Innovations in Glaucoma Study (DIGS) were prospectively included. FAF scans were obtained with the Spectralis HRA+OCT and CSLO reflectance images with the HRTII (both from Heidelberg Engineering, Heidelberg, Germany). Two masked graders independently measured β-PPA area on 3 consecutive scans using the semi-automated BluePeak RegionFinder software (BPRF) and on CSLO reflectance images using the optic disc contour line. Reproducibility of β-PPA area measurements was assessed using intraclass correlation coefficients (ICC). Results: Intragrader reproducibility was 0.997 (95% CI, 0.996-0.998) and 0.995 (95% CI, 0.992-0.996) for grader 1 and 2, respectively, using FAF-BPRF, and by CSLO, it was 0.991 (95% CI, 0.986-0.994) and 0.988 (95% CI, 0.982-0.992). Intergrader agreement (ICC) was 0.53 (95% CI, 0.331-0.685) for FAF-BPRF and 0.404 (95% CI, 0.149-0.601) for CSLO (comparison between ICC, p = 0.368). Agreement (ICC) between the two devices was worse for grader 1 (0.356; 95% CI, 0.129-0.549) than grader 2 (0.856; 95% CI, 0.774-0.910) (p < 0.001). Conclusions: Despite excellent intragrader reproducibility for β-PPA measurements with FAF-BPRF and CSLO, intergrader reproducibility is low to moderate. Measurements of β-PPA area obtained with the two instruments are of moderate agreement and, therefore, are not interchangeable.

Original languageEnglish (US)
JournalActa Ophthalmologica
Volume91
Issue number5
DOIs
StatePublished - Aug 1 2013
Externally publishedYes

Fingerprint

Ophthalmoscopy
Atrophy
Lasers
Light
Software
Glaucoma
Optic Disk
Reproducibility of Results
Germany
Equipment and Supplies

Keywords

  • β-zone
  • blue-peak autofluorescence
  • glaucoma
  • parapapillary atrophy

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Semiautomated quantification of β-zone parapapillary atrophy using blue light fundus autofluorescence. / Mansouri, Kaweh; Tung, Jonathan D.; Medeiros, Felipe A.; Tafreshi, Ali; Dorairaj, Syril; Zangwill, Linda; He, Feng; Jain, Sonia; Weinreb, Robert N.

In: Acta Ophthalmologica, Vol. 91, No. 5, 01.08.2013.

Research output: Contribution to journalArticle

Mansouri, K, Tung, JD, Medeiros, FA, Tafreshi, A, Dorairaj, S, Zangwill, L, He, F, Jain, S & Weinreb, RN 2013, 'Semiautomated quantification of β-zone parapapillary atrophy using blue light fundus autofluorescence', Acta Ophthalmologica, vol. 91, no. 5. https://doi.org/10.1111/aos.12057
Mansouri, Kaweh ; Tung, Jonathan D. ; Medeiros, Felipe A. ; Tafreshi, Ali ; Dorairaj, Syril ; Zangwill, Linda ; He, Feng ; Jain, Sonia ; Weinreb, Robert N. / Semiautomated quantification of β-zone parapapillary atrophy using blue light fundus autofluorescence. In: Acta Ophthalmologica. 2013 ; Vol. 91, No. 5.
@article{a9ded54d43d540408dbcecf53dd7bc50,
title = "Semiautomated quantification of β-zone parapapillary atrophy using blue light fundus autofluorescence",
abstract = "Purpose: To evaluate the reproducibility of measurements of area of β-zone parapapillary atrophy (β-PPA) using blue laser fundus autofluorescence (FAF) and confocal scanning laser ophthalmoscopy reflectance (CSLO) measurements and to assess agreement between the two imaging modalities. Methods: Sixty-five eyes of 45 patients (mean age, 68.2 ± 11.3 years) with established or suspected glaucoma from the Diagnostic Innovations in Glaucoma Study (DIGS) were prospectively included. FAF scans were obtained with the Spectralis HRA+OCT and CSLO reflectance images with the HRTII (both from Heidelberg Engineering, Heidelberg, Germany). Two masked graders independently measured β-PPA area on 3 consecutive scans using the semi-automated BluePeak RegionFinder software (BPRF) and on CSLO reflectance images using the optic disc contour line. Reproducibility of β-PPA area measurements was assessed using intraclass correlation coefficients (ICC). Results: Intragrader reproducibility was 0.997 (95{\%} CI, 0.996-0.998) and 0.995 (95{\%} CI, 0.992-0.996) for grader 1 and 2, respectively, using FAF-BPRF, and by CSLO, it was 0.991 (95{\%} CI, 0.986-0.994) and 0.988 (95{\%} CI, 0.982-0.992). Intergrader agreement (ICC) was 0.53 (95{\%} CI, 0.331-0.685) for FAF-BPRF and 0.404 (95{\%} CI, 0.149-0.601) for CSLO (comparison between ICC, p = 0.368). Agreement (ICC) between the two devices was worse for grader 1 (0.356; 95{\%} CI, 0.129-0.549) than grader 2 (0.856; 95{\%} CI, 0.774-0.910) (p < 0.001). Conclusions: Despite excellent intragrader reproducibility for β-PPA measurements with FAF-BPRF and CSLO, intergrader reproducibility is low to moderate. Measurements of β-PPA area obtained with the two instruments are of moderate agreement and, therefore, are not interchangeable.",
keywords = "β-zone, blue-peak autofluorescence, glaucoma, parapapillary atrophy",
author = "Kaweh Mansouri and Tung, {Jonathan D.} and Medeiros, {Felipe A.} and Ali Tafreshi and Syril Dorairaj and Linda Zangwill and Feng He and Sonia Jain and Weinreb, {Robert N.}",
year = "2013",
month = "8",
day = "1",
doi = "10.1111/aos.12057",
language = "English (US)",
volume = "91",
journal = "Acta Ophthalmologica",
issn = "1755-375X",
publisher = "Wiley-Blackwell",
number = "5",

}

TY - JOUR

T1 - Semiautomated quantification of β-zone parapapillary atrophy using blue light fundus autofluorescence

AU - Mansouri, Kaweh

AU - Tung, Jonathan D.

AU - Medeiros, Felipe A.

AU - Tafreshi, Ali

AU - Dorairaj, Syril

AU - Zangwill, Linda

AU - He, Feng

AU - Jain, Sonia

AU - Weinreb, Robert N.

PY - 2013/8/1

Y1 - 2013/8/1

N2 - Purpose: To evaluate the reproducibility of measurements of area of β-zone parapapillary atrophy (β-PPA) using blue laser fundus autofluorescence (FAF) and confocal scanning laser ophthalmoscopy reflectance (CSLO) measurements and to assess agreement between the two imaging modalities. Methods: Sixty-five eyes of 45 patients (mean age, 68.2 ± 11.3 years) with established or suspected glaucoma from the Diagnostic Innovations in Glaucoma Study (DIGS) were prospectively included. FAF scans were obtained with the Spectralis HRA+OCT and CSLO reflectance images with the HRTII (both from Heidelberg Engineering, Heidelberg, Germany). Two masked graders independently measured β-PPA area on 3 consecutive scans using the semi-automated BluePeak RegionFinder software (BPRF) and on CSLO reflectance images using the optic disc contour line. Reproducibility of β-PPA area measurements was assessed using intraclass correlation coefficients (ICC). Results: Intragrader reproducibility was 0.997 (95% CI, 0.996-0.998) and 0.995 (95% CI, 0.992-0.996) for grader 1 and 2, respectively, using FAF-BPRF, and by CSLO, it was 0.991 (95% CI, 0.986-0.994) and 0.988 (95% CI, 0.982-0.992). Intergrader agreement (ICC) was 0.53 (95% CI, 0.331-0.685) for FAF-BPRF and 0.404 (95% CI, 0.149-0.601) for CSLO (comparison between ICC, p = 0.368). Agreement (ICC) between the two devices was worse for grader 1 (0.356; 95% CI, 0.129-0.549) than grader 2 (0.856; 95% CI, 0.774-0.910) (p < 0.001). Conclusions: Despite excellent intragrader reproducibility for β-PPA measurements with FAF-BPRF and CSLO, intergrader reproducibility is low to moderate. Measurements of β-PPA area obtained with the two instruments are of moderate agreement and, therefore, are not interchangeable.

AB - Purpose: To evaluate the reproducibility of measurements of area of β-zone parapapillary atrophy (β-PPA) using blue laser fundus autofluorescence (FAF) and confocal scanning laser ophthalmoscopy reflectance (CSLO) measurements and to assess agreement between the two imaging modalities. Methods: Sixty-five eyes of 45 patients (mean age, 68.2 ± 11.3 years) with established or suspected glaucoma from the Diagnostic Innovations in Glaucoma Study (DIGS) were prospectively included. FAF scans were obtained with the Spectralis HRA+OCT and CSLO reflectance images with the HRTII (both from Heidelberg Engineering, Heidelberg, Germany). Two masked graders independently measured β-PPA area on 3 consecutive scans using the semi-automated BluePeak RegionFinder software (BPRF) and on CSLO reflectance images using the optic disc contour line. Reproducibility of β-PPA area measurements was assessed using intraclass correlation coefficients (ICC). Results: Intragrader reproducibility was 0.997 (95% CI, 0.996-0.998) and 0.995 (95% CI, 0.992-0.996) for grader 1 and 2, respectively, using FAF-BPRF, and by CSLO, it was 0.991 (95% CI, 0.986-0.994) and 0.988 (95% CI, 0.982-0.992). Intergrader agreement (ICC) was 0.53 (95% CI, 0.331-0.685) for FAF-BPRF and 0.404 (95% CI, 0.149-0.601) for CSLO (comparison between ICC, p = 0.368). Agreement (ICC) between the two devices was worse for grader 1 (0.356; 95% CI, 0.129-0.549) than grader 2 (0.856; 95% CI, 0.774-0.910) (p < 0.001). Conclusions: Despite excellent intragrader reproducibility for β-PPA measurements with FAF-BPRF and CSLO, intergrader reproducibility is low to moderate. Measurements of β-PPA area obtained with the two instruments are of moderate agreement and, therefore, are not interchangeable.

KW - β-zone

KW - blue-peak autofluorescence

KW - glaucoma

KW - parapapillary atrophy

UR - http://www.scopus.com/inward/record.url?scp=84880285576&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84880285576&partnerID=8YFLogxK

U2 - 10.1111/aos.12057

DO - 10.1111/aos.12057

M3 - Article

C2 - 23506312

AN - SCOPUS:84880285576

VL - 91

JO - Acta Ophthalmologica

JF - Acta Ophthalmologica

SN - 1755-375X

IS - 5

ER -