Semaphorin III can repulse and inhibit adult sensory afferents in vivo

Darrell L. Tanelian, Michael A Barry, Stephen Albert Johnston, Thuy Le, George M. Smith

Research output: Contribution to journalArticle

103 Citations (Scopus)

Abstract

During development, semaphorins (collapsin, fasciclin) mediate repulsive and inhibitory guidance of neurons. Semaphorin III, a secretable member of this fatally, is expressed by the ventral spinal cord at the time corresponding to projection of sensory afferents from the dorsal root ganglion (DRG) into the spinal cord. The inhibitory effect of E14 ventral cord is active only on nerve growth factor (NGF)-responsive sensory afferents (small-diameter A-delta and C fibers subserving sensations of temperature and pain). Similarly, COS cells secreting recombinant semaphorin III are able to selectively repel DRG afferents whose growth is stimulated by NGF and not NT- 3 (ref. 7). However, it is not known whether these molecules can exert a functional role in the fully developed adult peripheral nervous system. In this study, we demonstrated that gene gun transfection and production of semaphorin III in corneal epithelial cells in adult rabbits in vivo can cause repulsion of established A-delta and C fiber trigeminal sensory afferents. In addition, it is shown that, following epithelial wounding and denervation, semaphorin III is able to inhibit collateral nerve sprouts from innervating the reepithelialized tissue. These findings are significant in that they provide direct evidence that small-diameter adult sensory neurons retain the ability to respond to semaphorin III. In addition, the corneal gene gun technique may be generally used to study the in vivo effects of neural growth modulators by quantifying the amount of sensory nerve growth.

Original languageEnglish (US)
Pages (from-to)1398-1401
Number of pages4
JournalNature Medicine
Volume3
Issue number12
DOIs
StatePublished - Dec 1997
Externally publishedYes

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Semaphorins
Unmyelinated Nerve Fibers
Spinal Ganglia
Nerve Growth Factor
Neurons
Spinal Cord
Growth
Genes
Biolistics
Semaphorin-3A
Aptitude
Fibers
COS Cells
Peripheral Nervous System
Firearms
Neurology
Denervation
Sensory Receptor Cells
Modulators
Transfection

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Semaphorin III can repulse and inhibit adult sensory afferents in vivo. / Tanelian, Darrell L.; Barry, Michael A; Johnston, Stephen Albert; Le, Thuy; Smith, George M.

In: Nature Medicine, Vol. 3, No. 12, 12.1997, p. 1398-1401.

Research output: Contribution to journalArticle

Tanelian, DL, Barry, MA, Johnston, SA, Le, T & Smith, GM 1997, 'Semaphorin III can repulse and inhibit adult sensory afferents in vivo', Nature Medicine, vol. 3, no. 12, pp. 1398-1401. https://doi.org/10.1038/nm1297-1398
Tanelian, Darrell L. ; Barry, Michael A ; Johnston, Stephen Albert ; Le, Thuy ; Smith, George M. / Semaphorin III can repulse and inhibit adult sensory afferents in vivo. In: Nature Medicine. 1997 ; Vol. 3, No. 12. pp. 1398-1401.
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